Laminin is an extracellular matrix multi-domain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. The optimal concentration for cell culture depends on cell type and specific application.
Alternatively, a ready-to-use solution of poly-D-lysine and laminin is available for use with neural stem cells.
Laminin and fibronectin are widely used extracellular matrix (ECM) proteins; however, for best results, experimentally determine the ideal ECM for each cell line. Stem cells express varied integrins on the surface during different stages of differentiation. The choice of ECM protein depends on the stage of differentiation you are studying.
We do not determine the purity of laminin solutions. An SDS-PAGE gel is performed and the pattern (three bands) is comparable to past lots to ensure consistent quality.
The solvents that are compatible with dilute laminins are provided in Table 1.
Reuse of laminin is not recommended as there will be changes in the sterility and concentration and structure of laminin. Diluted, unused laminin can be stored at 2 - 8 °C for up to 1 week. Laminin-coated plates can be stored at 2 - 8 °C for up to 4 weeks provided they are sealed well to prevent contamination and/or drying. Do not use the product if discoloration or web-like formations appear on the surface of the coated material.
The coating concentration of our laminins is suggested in Table 1. For best results, optimize the concentrations for your specific cells.
Laminin or fibronectin along with poly-D-lysine or poly-L-lysine support in vitro neural differentiation well. Refer to the protocol for detailed steps to induce neural differentiation.
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