DNA and RNA purification

Applied Filters:

facet applications:DNA and RNA purification

facet content type:Technical Article

Showing 1-30 of 37 results

The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of

RNA Isolation from Blood, Cultured Cells, and Animal or Plant Tissue on Whatman™ FTA™ Cards

This article describes the evaluation of Whatman FTA cards from Cytiva for their ability to collect, store, and isolate high-quality RNA from a variety of crude biological samples.

Downstream Applications after Genomic DNA Purification

This page shows downstream applications after genomic DNA purification.

Plasmid DNA Preparation Troubleshooting

This page shows possible causes and solutions for problems that may occur during plasmid DNA preparation.

Procedure for Purifying and Concentrating DNA using illustra™ GFX 96 PCR Purification Kit

This page shows the procedure for purifying and concentrating DNA using illustra™ GFX 96 PCR Purification Kit from Cytiva.

Oligonucleotide Array CGH Analysis of a Robust Whole Genome Amplification Method

In recent years, array-based Comparative Genomic Hybridization (aCGH) has been refined to determine chromosomal changes at progressively higher resolutions. This evolving technology is, however, somewhat hampered by the large DNA input requirement—a minimum of 150,000 copies of a human genome

Viral RNA Extraction Buffer for SARS-CoV-2 in Saline and Saliva

Viral RNA Extraction Buffer (VRE100) and related reagents for qRT-PCR and additional viral RNA analyses.

Complete Solutions for PCR Assay Development

Fit-for-use products offer the quality, consistency & documentation necessary for every step of your IVD development and manufacturing process.

Benzonase^® Nuclease for Microbiome Workflows

Benzonase® Nuclease for reducing host DNA in microbiome workflows and enhancing taxa identification.

MetaPolyzyme, DNA Free Cell Lysis Enzymes for Microbiome Workflows | Sigma-Aldrich

Explore the benefits of MetaPolyzyme, DNA free to improve DNA extraction and increase the number of taxa identified in treated samples.

How to Determine the Best Product & Supplier for Proteinase K

The cost of proteinase K can vary depending on the source, purity, manufacturing process and vendor’s quality management system. It is important to balance the cost with the desired quality, performance, documentation and technical/quality support to select the optimal Proteinase

Guide on How to Use Proteinase K in Different Procedures

Guidelines on use of proteinase K, an enzyme commonly used to degrade proteins, and protect DNA and RNA from degradation in samples.

Impact of GenElute™-E Purification Method on Accuracy of DNA Quantitation and Downstream Enzymatic Processes

Impact of Purification Method on Accuracy of DNA Quantitation and Downstream Enzymatic Processes. Evaluation of the purity of genomic DNA by UV spectrophotometry, gel electrophoresis, and downstream qPCR using GenElute™-E DNA purification kits.

Water for Nuclease-sensitive Molecular Biology Studies

Nuclease-free water (or PCR water) is needed in molecular biology to avoid sample degradation during PCR, sequencing, etc. Ultrafiltration provides a quicker, safer, and more sustainable alternative to DEPC treatment when preparing RNase-free and DNase-free water.

Extract-N-Amp™ Tissue Feature Article

The Extract-N-Amp™ Tissue PCR Kit has been created to release PCR-ready DNA from mouse tails in a 15 minute single tube procedure. The included 2x PCR mix is optimized to work with the crude extracts and the solutions in the

KAPA Express Extract Kit FAQs

Extract DNA from crude samples for PCR applications, including qPCR, using the KAPA Express Extract Kit. See frequently asked questions regarding sample type, optimization and storage.

Oligonucleotide Purification

Discover the best purification option for your oligo, based on the intended application.

How Proteinase K Can Be Used in Tissue Samples

The use of Pro K in combination with other reagents, such as detergents and chaotropic agents, can help to disrupt the cell membranes and release DNA from tissue. This is particularly important for downstream applications such as PCR, sequencing, and

Long-term Storage of Blood in DNAgard® Blood

Two separate studies were conducted to analyze long-term storage of blood in DNAgard Blood stabilization reagent.

Biomatrica® offers an innovative alternative to reliance on cold chain logistics

Biomatrica® products enable researchers to stabilize, ship, and store biological samples for long periods of time.

Extract-N-Amp™ Blood PCR Kit Protocol

The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

Extract-N-Amp Reagent: Effective PCR from FTA® Blood Cards

Extract-N-Amp simplifies DNA extraction and amplification, enabling PCR directly from FTA blood cards, reducing variability.

Stabilizing RNA at room temperature in RNAstable®

RNAstable® is a novel preservation product developed to protect RNA from degradation during storage or shipment at ambient temperatures. The synthetic storage medium is based on the natural principles of anhydrobiosis (meaning “life without water”), a biological mechanism employed by

Sodium acetate buffer solution for molecular biology

Sodium Acetate Buffer provider. 5+ decades providing high-quality buffer products that maintain consistent performance for molecular biology .

Protein Affinity Chromatography

Affinity chromatography is the process of bioselective adsorption and subsequent recovery of a compound from an immobilized ligand. This process allows for the highly specific and efficient purification of many diverse proteins and other compounds. The process requires

High Pure Technology and Silica Adsorption Kits

The High Pure and silica adsorption kits developed by Roche depend on the tendency of nucleic acids to adsorb to silica (glass) in the presence of a chaotropic salt such as sodium iodide (NaI), guanidine thiocyanate or guanidine hydrochloride [Melzak

Oligonucleotide Quality Control by Analytical HPLC

In many cases oligonucleotides require quantitative analysis to verify specifications have been met. MALDI-TOF and ESI-MS can provide qualitative-quantification for purity.

ECACC Nucleic Acids

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

Sera-Mag™ and Sera-Mag SpeedBeads Magnetic Particles

Sera-Mag and Sera-Mag SpeedBeads provide cost effective magnetic bead separation technology for molecular biology applications, nucleic acid isolation, and research immunoassays.

Page 1 of 2