Gene expression and silencing

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Showing 1-18 of 18 results

Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents

A detailed protocol for the transduction of human embryonic stem cells using Lentiviral Vectors. Includes product list and references.

Preparation of the Lentiviral Transduction Particles Using Packaging Plasmid Mix

Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit®

This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.

Escort™ IV Transfection Reagent Protocol

Product manual provides detailed protocol for easy DNA transfection.

Lentiviral Transduction Protocol

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

X-tremeGENE™ HP DNA Transfection Reagent Protocol

Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

Biodegradable Nanoparticles

Preparation for biodegradable nanoparticles and their use in transfection protocols .

CRISPR Lentiviral Screening: Antibiotic Selection

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

MISSION® shRNA Guarantee

Guarantee efficient gene knockdown with our shRNA clones, ensuring reliable results for targeted transcript suppression.

Genome Editing in Plants with CRISPR/Cas9

ZFNs and CRISPR/Cas9 advance targeted genome editing, opening new research avenues.

FACS Titration of Lentivirus Protocol

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

Spinoculation Protocol

Protocol for lentiviral transduction of suspension cells using MISSION TRC shRNA Lentiviral Particles for long-term silencing.

X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting

Preparation of Cationic Liposomes & Transfection of Cells - Avanti® Polar Lipids

This procedure describes the Preparation of Cationic Liposomes & Transfection of Cells

CRISPR Cas 9 Nuclease RNA-guided Genome Editing

Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.

Antibiotic Optimization for Cell Transduction

Determine optimal antibiotic concentration for stable cell lines, ensuring efficient selection in transduced cells.