Large molecule HPLC

Applied Filters:

facet applications:Large molecule HPLC

facet applications:Low pressure liquid chromatography

facet applications:Small molecule HPLC

facet content type:Technical Article

Showing 1-30 of 198 results

Analytical Size-exclusion Chromatography Columns for Biomolecule Separations

Informative article on size exclusion chromatography columns for biomolecule separations. Using columns packed with sub-2 μm particles provides significant gains in efficiency over SEC columns packed with larger particles.

HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein

Benefits of Ascentis Express Phenyl-Hexyl over Biphenyl for the Separation of Pain Management Opiates

Although both biphenyl and phenyl-hexyl phases can resolve these compounds, the former exhibits excellent peak shape and substantially less silanol-derived ion exchange activity.

HPLC Analysis of Sugars on Ascentis® Si

Separation of Sucrose; Maltose; Maltotriose

HPLC Analysis of Caseins on BIOshell™ A400 Protein C4

-casein basis (electrophoresis), lyophilized powder; β-Casein from bovine milk, BioUltra, ≥98% (PAGE)

Ultra-High Performance Liquid Chromatography (UHPLC) with the Ascentis® Express Phenyl-Hexyl Column

UHPLC Analysis of Cannabinoids on Ascentis Express C18

As the popularity of cannabis-infused products increases, there is a growing need to characterize the type and content of the cannabinoids found in the product. This application demonstrates the ability of the Ascentis Express C18 column to baseline resolve

Improved Analysis of Simple Sugars Using apHera™ NH2 HPLC Columns

The apHera NH2 polymeric column is an excellent choice for the separation of closely-related polar analytes such as simple sugars using HILIC chromatography.

Ascentis® Express Phenyl Hexyl Columns for U/HPLC

Improve your reversed phase selectivity for polar aromatics and heterocyclic compounds with the Ascentis® Express Phenyl-Hexyl U/HPLC column.

Ion Pairing for Analysis of Phosphonate Compounds

Phosphonates are a class of compounds commonly used for therapeutic applications of antiviral drugs. These compounds can be difficult to analyze due to their highly polar nature and lack of UV active chromophores.

An LC/MS Peptide Standard for Rapid System Suitability Assessment and Retention Time Translation Across LC/MS Platforms

Standards are critical in liquid chromatography/mass spectrometry (LC/MS) based proteomics to ensure optimal and consistent system performance before, during, and after sample analysis.

LC/MS/MS Method for Determination of Glyphosate, AMPA, and Glufosinate in Cereals

Glyphosate and related compounds are measured in oatmeal and infant cereal using ion-exchange polymer-based particles for HPLC and SPE. Low level detection was obtained.

Reference Standards for Analyzing Polyphenol Catechins

Two Distinct Sample Prep Approaches to Overcome Matrix Effect in LC/MS of Serum or Plasma Samples

This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.

Multiplex Quantification of Infliximab and Adalimumab in Human Serum by LC-MS/MS Using Full-Length Stable Isotope Labeled Internal Standards

Infliximab and Adalimumab monoclonal antibodies (mAbs) are used widely to treat rheumatoid arthritis, psoriatic arthritis, and many autoimmune diseases by binding to tumor necrosis factor-alpha (TNFα) to reduce the inflammatory response.

Impact of Mobile Phase Additives on LC-MS Sensitivity, Demonstrated using Spice Cannabinoids

This study demonstrates the impact of various mobile phase modifiers on the separation; formate modifiers outperform acetate in terms of MS signals (or sensitivity) and chromatographic resolution.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Maintenance of Multimodal Chromatography Media and Storage Conditions

This page describes the maintenance of media and storage conditions for multimodal chromatography using Cytiva products.

Purification or Removal of Proteins and Peptides

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.

Polishing of MAbs Using Capto Adhere ImpRes in Bind/Elute Mode

In these studies, the binding capacity for MAbs and the efficiency in the clearance of impurities using Capto adhere ImpRes in bind/elute mode was evaluated.

Capto MMC

Capto MMC is a multimodal cation exchanger with the properties of a weak cation exchanger. In addition to electrostatic interactions, the ligand structure provides for additional interaction modes such as hydrophobic interaction, hydrogen bonding, and thiophilic interaction.

Purification using GST SpinTrap™

This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.

Principles and Standard Conditions for Different Purification Techniques

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.

Purification or Removal of Viruses including Adeno-associated Virus

This page shows how to purify or remove viruses with a Capto DeVirS, AVB Sepharose High Performance from Cytiva.

Bind/Elute vs Flowthrough Mode in Multimodal Chromatography

This page describes the difference between bind/elute and ﬂowthrough modes in multimodal chromatography using Cytiva products.

Bioethanol

Learn more about Bioethanol as a renewable, alternative fuel traditionally produced by the yeast fermentation of sugar.

Absolute Quantification of Serum Proteome

Absolute Quantification (Protein-AQUA™™) is a targeted quantitative proteomics technique that exhibits robust efficacy and is being increasingly utilized for a wide variety of quantitative proteomics studies.

BIOshell™ A400 Protein C4 U/HPLC Columns

BIOshell™ A400 Protein C4 columns contain 3.4 μm particles with 400 Å pores, which are derivatized with dimethylbutyl silane and exhaustively endcapped for optimum protein recovery.

Improving the Chromatographic Separation of DMB-Labeled Sialic Acids for the Comparison of Biosimilars to Reference Materials

A significantly improved HPLC-fluorescence method for DMB-NANA and -NGNA, and application of this method to compare 2 candidate biosimilar therapeutic proteins to their respective RMs.

Rapid Trypsin Digestion of Complex Protein Mixtures for Proteomics Analysis

Reliable and reproducible results in mass spectrometry proteomics analyses are highly dependent on robust sample preparation.

Page 1 of 7