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Mammalian cell culture
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Hematopoietic Cytokines
Read article on hematopoietic cytokines and hematopoiesis
F-12 Coon's Modification Formulation
F-12 Coon's Modification Formulation
L-15 Media Formulation
L-15 Media Formulation
Iscove's Modified Dulbecco's Media (IMDM) Formulation
Iscove's Modified Dulbecco's Media (IMDM) Formulation
Cyclodextrins
Many metabolically important compounds, such as lipid-soluble vitamins and hormones, have very low solubilities in aqueous solutions. Various techniques have been used to solubilize these compounds in tissue culture, cell culture, or other water-based applications.
Cell Analysis Best Practices
How do you assess the state of your cell culture? We’ve got some best practices to share.
Cultivating Consistency: Tips and Tricks for Successful Cell Cultures
Properly optimizing workflows and utilizing the latest best practices are the keys to obtaining consistent results and advancing research.
Cell Freezing and Banking
Cell Freezing and Banking
Fibroblast Growth Factor Family (FGF)
Fibroblast growth factors in cell culture and various growth factors for your research
Oxidative Stress in Cell Culture
Read how oxygen, oxygen free radicals, oxidative stress and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing of monoclonal antibodies
Transferrin in Cell Culture
How transferrin and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of transferrin.
Counting of Cells
Trypan blue method of cell counting is inexpensive and readily available method. The method is based on principle that living cells exclude trypan blue dye and appear bright, while dead cells take up dye and appear blue. Watch video to
VEGF Pathway
Read review on VEGF signaling pathway (Vascular endothelial growth factor (VEGF)/VEGF receptor) and find related products.
Cell Culture Protocol 10: Testing Cells for Mycoplasma Contamination by Hoechst DNA Staining
Cell culture protocol for testing cell lines for mycoplasma contamination using indirect DNA staining using Hoechst 33342.
Autophagy Mitophagy in Tumor Cell Survival Death
Autophagy is a highly regulated process that is involved in cell growth, development, and death. In autophagy cells destroy their own cytoplasmic components in a very systematic manner and recycle them.
Autophagy in Cancer Promotes Therapeutic Resistance
We presents an article on Autophagy in Cancer Promotes Therapeutic Resistance
Fatty Acid Synthesis and Metabolism in Cancer Cells
Information on fatty acid synthesis and metabolism in cancer cells. Learn how proliferatively active cells require fatty acids for functions such as membrane generation, protein modification, and bioenergetic requirements. These fatty acids are derived either from dietary sources or are
Lipid-induced Insulin Resistance
Lipid Induced Insulin Resistance
NF-κB and Inflammation
Chronic inflammation is an underlying factor in the development and progression of many of the chronic diseases of aging, such as arthritis, atherosclerosis, diabetes, and cancer.
Alternative High-Yield Cell Culture Systems
Solutions for high-yield cell culture scale-up applications including multi-layered flasks (HyperFlasks/CellStacks), roller bottles, spinner and shaker flasks. Free ECACC handbook download.
Cell Types & Culture Characteristics
An overview of how to classify various cell types (immortalized, primary or stem cells) based on cellular morphology characteristics such as epithelial, endothelial, neuronal or fibroblast. Cell growth characteristics also discussed. Free ECACC handbook download.
How to Fractionate and Analyze Gradients of Percoll
Density Marker Beads are dyed derivatives of Sephadex™. There are ten color-coded bead types, each with a specific density. They have been specifically formulated for use in Percoll gradients and will not work with other media. Using Density Marker Beads
Removal of Percoll after Centrifugation
This page shows methods to remove Percoll from a sample after centrifugation.
Identification of Cell Culture Media with Raman Spectroscopy
Identification of cell culture media samples by traditional LC methods, such as amino acid or vitamin analysis, has high costs, and requires significant analytical expertise and laboratory space.
Cell Culture Fundamentals: Cryopreservation and Storage of Cell Lines
Overview on liquid nitrogen storage and freezing of cell stocks for cell line cryopreservation purposes. Free ECACC handbook download.
Hybridoma Fusion Partners Cell Lines
Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.
3D Neurosphere Culture Media
3D neurosphere culture protocol to expand human U-87 MG glioblastoma cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.
Linolenic Acid in Cell Culture
Importance and uses of linolenic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Linoleic acid in Cell Culture
Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Cell Clumping Troubleshooting
Common Cell Culture Problems: Cell Clumping -- Cells in suspension may attach to one another and form clumps for a variety of reasons. The most common cause of cell clumping is the presence of free DNA and cell debris in
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