Merck
CN
Advanced Search
Chemical Structure Search
Search Within
Applications
Content Type

Primary cell culture

Applied Filters:
facet applications:Primary cell culture
facet content type:Protocol
Showing 1-30 of 40 results
Protocol for Preparation and Cultivation of PromoCell® Cryopreserved and Proliferating Cells
Protocol for the seeding and cultivation of primary cells received in cryopreserved or proliferating formats. Information on primary cell culture, cell handling and passaging.
Human Aortic Endothelial Cells (HAOEC) Culture Protocol
Technical information for working with human aortic endothelial cells including thawing, subculturing and cryopreservation.
Protocol Guide: XTT Assay for Cell Viability and Proliferation
XTT assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for XTT reagent preparation and examples of applications.
Trilineage Differentiation of Multipotent Human Mesenchymal Stem Cells (MSCs) into Osteocytes, Adipocytes and Chondrocytes
Osteogenesis, adipogenesis and chondrogenesis differentiation protocols and media for human mesenchymal stem cell culture. Explore over 350 PromoCell products on our company website.
In Vitro Expansion of CD34+/CD38- Human Hematopoietic Progenitor Cells in a Serum-free and Xeno-free Stem Cell Culture Media
In vitro culture of human hematopoietic stem cells in PromoCell’s serum-free/xeno-free HPC Expansion Medium DXF. Explore over 350 PromoCell products
ECM Coating Protocols for Millicell®
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
Human Mesenchymal Stem Cells and Growth/Differentiation Media
Mesenchymal Stem Cells and Media were developed for the in vitro expansion and directed differentiation of mesenchymal stem cells (MSC) from bone marrow, the umbilical cord matrix (Wharton´s Jelly) and adipose tissue. Protocols for handling, cell passaging, media, and
Primary Cell Culture FAQ
Primary Cell Culture: Frequently Asked Questions (FAQs)
In Vitro Differentiation of Human PBMC Derived Monocytes into M1 or M2 Macrophages in a Serum-free and Xeno-free Cell Culture Media
Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.
Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol
Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol. Storage. Preparation for Culturing. Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Lung Fibroblasts (HLF) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Epidermal Keratinocytes (HEK) Culture Protocol
Storage - Preparation for Culturing - Culturing HEK - Subculturing HEK
Human Skeletal Muscle Cells (HSkMC) Culture Protocol
Technical information for working with human skeletal muscle cells including thawing, subculturing and cryopreservation
Rat Skeletal Muscle Cells (RSkMC) Culture Protocol
Technical information for working with rat skeletal muscle cells including thawing, subculturing and cryopreservation
Rat Brain Microvascular Endothelial Cells (RBMVEC) Culture Protocol
Technical information for working with rat brain microvascular endothelial cells including thawing, subculturing and cryopreservation
Human Coronary Artery Endothelial Cells (HCAEC) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Human Chondrocytes (HC) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival. Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room
Ficoll>sup>® 400 for Gradient Centrifugation
Ficoll 400® is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400® is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400® is very hydrophilic and extremely water-soluble.
Primary Human Hepatocytes Culture Protocol
Discover our primary human hepatocytes and plating and maintenance media formulations. Explore our protocol for plateable primary hepatocyte cell culture.
Primary Human Hepatic Sinusoidal Endothelial Cells Culture Protocol
Discover our collection of primary human hepatic sinusoidal endothelial cells, a protocol for thawing, plating, and growing HHSECs, and a recipe for our HHSEC maintenance media formulation.
Primary Human Hepatic Kupffer Cell Culture Protocol
Discover our collection of primary human hepatic Kupffer cells and protocol for thawing, plating, and growing Kupffer cells. Find the formulation for Kupffer culture maintenance media.
Primary Human Spheroid-Qualified Plateable Hepatocyte Culture Protocol
Discover our protocol for thawing, plating, and forming hepatocyte spheroids from human spheroid-qualified plateable hepatocytes. Find the formulation for hepatocyte spheroid maintenance media.
Human Epidermal Melanocytes (HEM) Culture Protocol
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Culturing Human Aortic Smooth Muscle Cells
Technical information for working with human aortic smooth muscle cells including thawing, subculturing and cryopreservation.
In Vitro Differentiation of Human PBMC and CD34+ Derived Monocytes into Mature CD83+/CD14- Dendritic Cells
Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.
ADCC Protocol using BioIVT Cryopreserved NK Effector Cells
Step-by-step protocol for ADCC assays using cryopreserved NK effector cells including tips and tricks, assay development, and data analysis methods.
Human Bronchial Epithelial Cell line
Technical information for working with human bronchial epithelial cell line including thawing, subculturing and cryopreservation.
Human Cardiac Fibroblasts (HCF) Culture Protocol
Isolated from adult human heart ventricles, human cardiac fibroblasts (HCF) are used to study myocardial infarction, cardiac fibrosis, and cardiac hypertrophy. They play a critical role in extracellular matrix maintenance, growth factor synthesis, and cytokine synthesis. This protocol outlines the
Human Dermal Fibroblasts (HDF) Culture Protocol
This protocol contains: Storage - Preparation for Culturing - Culturing HDF - Subculturing HDF
Human Follicle Dermal Papilla Cells (HFDPC) Culture Protocol
Technical information for working with human follicle dermal papilla cells including thawing, subculturing and cryopreservation.
Page 1 of 2