Protein biology

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Fragmentation of chromatin in Chromatin Immunoprecipitation (ChIP)

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Epidermal Growth Factor Receptor (EGFR) Signaling

Review EGF/EGFR signaling (epidermal growth factor receptor) and find links to related products.

Nuclear Receptors (Steroids)

We offers many products related to Nuclear Receptors (Steroids) for your research needs.

Enzyme Inhibitors Role

Learn the role of enzyme inhibitors including why enzyme inhibitors are important, the advantages of small molecule inhibitors vs RNAi, and how enzymes work with lock-and-key and induced fit models.

Automated IgG Purification Using PureProteom

PureProteome™ Protein A and G Magnetic beads provide a rapid and reproducible means to purify immunoglobulins (IgG) using the KingFisher Duo particle processor.

Calcitonin Gene-Related Peptides

Sigma-Aldrich offers many products related to calcitonin gene-related peptides for your research needs.

Prestige Antibodies® Organelle Markers

Subcellular localization studies are important for mapping and characterizing proteins and thus for better understanding of the cellular functions of the proteins. By confocal microscopy analysis on human cell lines, spatial and temporal protein expression patterns can be visualized on

GPCR Markers for Cancer Research

Explore the role of some selected GPCR markers in cancer research development and treatment, including neurohormonal GPCRs, rhodopsin-like GPCRs, and more.

Quantification of Host Cell Proteins

Residual presence of host cell proteins (HCPs) in recombinant therapeutic products has considerable clinical safety risks associated with a potential immunological response in patients.

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Crystallization of Biomolecules

Many soluble proteins, membrane proteins, nucleic acids and nucleoprotein complexes have been obtained in a crystalline form suitable for crystallographic investigation.

Protein AQUA™ Applications

Utilize the protein AQUA™ technique to measure expression of silenced genes, quantitate protein levels for low abundance serum proteins and analyze phosphorylated peptides enriched with immobilized metal affinity chromatography.

Sigma Receptors

Sigma receptors may constitute favorable targets for drug design in conditions such as psychiatric and movement disorders, amnesia, depression, cancer, inflammation and cocaine addiction.