Protein labeling and modification

Applied Filters:

facet applications:Protein labeling and modification

facet content type:Protocol

Showing 1-10 of 21 results

Purification or Removal of Biotin and Biotinylated Biomolecules with Magnetic Beads

This page shows how to Purify or remove biotin and biotinylated biomolecules with magnetic beads.

Calf Intestinal (CIP) Alkaline Phosphatase for Nucleic Acid Dephosphorylation

CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.

Alkaline Phosphatase Protocol

Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.

Clean-up of Antibody Labeling Reactions Using Amicon Ultra Filters

Using a standard Amicon® Ultra centrifugal filter, a few rounds of diafiltration can efficiently remove unincorporated label from an antibody labeling reaction without diluting the sample.

Conjugating Alkaline Phosphatase to Antibodies and Other Proteins

Protocol for the one-step, glutaraldehyde coupling method to conjugation alkaline phosphatase to antibodies and other proteins.

Coupling Protocol for Primary Amine of a Ligand

This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.

Coupling to Microparticles

Biochemicals Coupling to Microparticles. Besides Proteins, DNA, lectins and many other species have been coupled to microparticles.

Enzymatic Deglycosylation

Enzymatic Deglycosylation Strategies: Sequential hydrolysis of individual monosaccharides from glycans can be useful for the elucidation of the structure and function of the glycan component.

N-Glycosidase F Protocol & Troubleshooting

Neuraminidase (Sialidase) Protocol

Neuraminidase can be used to cleave sialic acids from proteins. In this protocol, the enzyme from Vibrio cholerae is used on fixed cells.

Page 1 of 3