Protein labeling and modification

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What is Mass Spectrometry or MS? What is it used for and how does it work? Read this detailed article with diagrams and example graphs to understand how to use mass spec in your research.

Near-infrared (NIR) Fluorescence Dyes

NIR Fluorescence Dyes

EX-CELL® Glycosylation Adjust (GAL+)

Our protein quality supplement, EX-CELL® Glycosylation Adjust (Gal+), provides customers with a novel chemically defined product which targets glycosylation attributes.

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification

ChiPros Chiral Amines

Chiral amines play an important role in stereoselective organic synthesis. They are used directly as resolving agents, building blocks, or chiral auxiliaries.

Troubleshooting Strategies in GST-tagged Protein Purification

This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.

Introduction to Target Identification with Hyas and Dione Kits

In cases where photoaffinity labeling (PAL) probes are ineffective, Hyas and Dione Kits provide an effective method for small molecule identification by using an Iridium photocatalyst tethered to the molecule under investigation.

Glycoprotein Deglycosylation

Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.

Peptidoglycan Structure and Biosynthesis

Peptidoglycan structure: alternating GlcNAc and N-acetylmuramic acid cross-linked to peptides form the basic backbone.

Phosphorylation Basics

Explore phosphorylation types converting ADP to ATP, comparing oxidative and substrate-level phosphorylation with explanatory diagrams.

Designing Peptides

Consider sequence length, solubility, and stability when designing peptides for custom synthesis.

Phosphorylation Analysis Tools

Phosphorylation's role in cell signaling pathways and protein modification.

Y-Shape Peg Derivatives in Drug Delivery

Immune system defends against disease by hydrolyzing foreign molecules; proteases, nucleases, liver, and kidneys are involved.

Glycosyltransferases

Enzymatic glycosyltransferase specificity challenges the one enzyme-one linkage concept.

Peptide Stability

Consider sequence length, solubility, and stability when selecting peptides for custom synthesis.

Glycosyltransferases: Tools for Synthesis and Modification of Glycans

Explore tools for glycosyltransferase synthesis and modification of glycans, such as glycosyltransferases and nucleotide sugar donors.

Peptide Coupling Reagents Guide

Novabiochem® coupling reagents for in situ activation are fast-reacting and compatible with various amino acids.

Glycosylphosphatidylinisotol-anchored Glycoproteins Overview

Glycosylphosphatidylinisotol- (GPI) anchored proteins have been identified throughout a broad range of eukaryotic species ranging from humans to insects, yeasts, bacteria, and fungi, suggesting that they are an ancient modification.

Ni-NTA-Atto Conjugates

Ni-NTA-Atto conjugates provide specific and highly sensitive detection of His-tagged fusion proteins.

ChemMatrix® Resin for solid phase peptide synthesis

ChemMatrix® is a proprietary, 100% PEG (polyethylene glycol) based resin from PCAS BioMatrix.

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

Self-Assembled Monolayers: Advantages of Pure Alkanethiols

We presents an article regarding Self-Assembled Monolayers: Advantages of Pure Alkanethiols.

Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression

Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression.

Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

Enzymes for Antibody Characterization

Antibodies now make up the fastest growing category of therapeutic drugs

Chiral Amines in Asymmetric Synthesis

Chiral amines have found widespread application in asymmetric synthesis serving, for instance, as chiral bases in enantioselective deprotonation reactions or being valuable substances for resolving racemic mixtures of acids.

Peptide Modifications: N-Terminal, Internal, and C-Terminal

Cleavage and Purification of GST-Tagged Protein Bound to GSTrap

This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.

Unnatural Amino Acids for Peptide Synthesis

Unnatural amino acids, the non-proteinogenic amino acids that either occur naturally or are chemically synthesized, are becoming more and more important as tools for modern drug discovery research.

N-Linked Glycans Overview

N-linked glycosylation, modification, and degradation

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