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Protein labeling and modification
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EX-CELL® Glycosylation Adjust (GAL+)
Our protein quality supplement, EX-CELL® Glycosylation Adjust (Gal+), provides customers with a novel chemically defined product which targets glycosylation attributes.
Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression
Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression.
Atto & Tracy Dyes for Protein Labeling
Protein labeling kits with Atto and Tracy dyes provide easy fluorescent labeling of purified proteins, enzymes, and antibodies.
GlycoProfile™ ß-Elimination Kit
Our GlycoProfile Beta-Elimination Kit enables comprehensive O-glycoproteomics research, overcoming challenges in complete O-deglycosylation.
Biotinylated Peptides
Streptavidin HC plates with high-capacity coating offer selective binding, reducing non-specificity.
Improving Glycoform Coverage for Mucin Prep
Human Protein Atlas uses A-431, U-251 MG, and U-205 cell lines with Prestige Antibodies for organelle mapping.
ChemMatrix® Resin for solid phase peptide synthesis
ChemMatrix® is a proprietary, 100% PEG (polyethylene glycol) based resin from PCAS BioMatrix.
Chymotrypsin
Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.
Designing Peptides
Consider sequence length, solubility, and stability when designing peptides for custom synthesis.
Enzymes for Antibody Characterization
Antibodies now make up the fastest growing category of therapeutic drugs
Glycan Labeling
Glycans play a key role in protein structure and disease; representation on cell surfaces is the glycome.
Glycoprofile™ Labeling Kits
Glycoprofile Labeling Kits efficiently label glycans for glycan analysis, offering flexibility in choice of labeling.
Glycoprotein Deglycosylation
Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.
Glycosyltransferases
Enzymatic glycosyltransferase specificity challenges the one enzyme-one linkage concept.
Glycosylphosphatidylinisotol-anchored Glycoproteins Overview
Glycosylphosphatidylinisotol- (GPI) anchored proteins have been identified throughout a broad range of eukaryotic species ranging from humans to insects, yeasts, bacteria, and fungi, suggesting that they are an ancient modification.
Introduction to Proximity Labeling
Novel method tags antibodies with photoreactive iridium for membrane protein labeling.
Introduction to Target Identification with Hyas and Dione Kits
In cases where photoaffinity labeling (PAL) probes are ineffective, Hyas and Dione Kits provide an effective method for small molecule identification by using an Iridium photocatalyst tethered to the molecule under investigation.
N-Linked Glycans Overview
N-linked glycosylation, modification, and degradation
Strategies for Deglycosylating N-Linked Glycans
Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
Near-infrared (NIR) Fluorescence Dyes
NIR Fluorescence Dyes
Ni-NTA-Atto Conjugates
Ni-NTA-Atto conjugates provide specific and highly sensitive detection of His-tagged fusion proteins.
Review of O-Linked Glycoproteins
O-Linked glycoproteins are usually large proteins with a molecular mass of >200 kDa. Glycosylation generally occurs in high-density clusters and may represent as much as 50-80% of the overall mass.
O Linked Glycans
O-Linked glycans attachment to peptides involves various residues, particularly GalNAc, known as mucin-type glycans.
Peptide Modifications: N-Terminal, Internal, and C-Terminal
Peptide Modifications: N-Terminal, Internal, and C-Terminal
Peptide Stability
Consider sequence length, solubility, and stability when selecting peptides for custom synthesis.
Peptidoglycan Structure and Biosynthesis
Peptidoglycan structure: alternating GlcNAc and N-acetylmuramic acid cross-linked to peptides form the basic backbone.
Phosphorylation Basics
Explore phosphorylation types converting ADP to ATP, comparing oxidative and substrate-level phosphorylation with explanatory diagrams.
Post Translational Modification
Protein modifications are crucial for disease study. Analysis methods are key.
Protein Deglycosylation
Protein Deglycosylation: PNGase F Proteomics Grade, Native Protein Deglycosylation Kit, Enzymatic Protein Deglycosylation Kit, and ProteoProfile Trypsin In-Gel Digest Kit
Phosphorylation Analysis Tools
Phosphorylation's role in cell signaling pathways and protein modification.
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