Search Within
Applications
Content Type
Protein labeling and modification
Applied Filters:
facet applications:Protein labeling and modification
Showing 1-30 of 66 results
Mass Spectrometry
What is Mass Spectrometry or MS? What is it used for and how does it work? Read this detailed article with diagrams and example graphs to understand how to use mass spec in your research.
Coupling to Microparticles
Biochemicals Coupling to Microparticles. Besides Proteins, DNA, lectins and many other species have been coupled to microparticles.
Near-infrared (NIR) Fluorescence Dyes
NIR Fluorescence Dyes
Conjugating Alkaline Phosphatase to Antibodies and Other Proteins
Protocol for the one-step, glutaraldehyde coupling method to conjugation alkaline phosphatase to antibodies and other proteins.
EX-CELL® Glycosylation Adjust (GAL+)
Our protein quality supplement, EX-CELL® Glycosylation Adjust (Gal+), provides customers with a novel chemically defined product which targets glycosylation attributes.
Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification
Tag Removal by Enzymatic Cleavage
This protocol shows how to remove histidine tags by enzymatic cleavage using Cytiva products.
Peptide Quick Tips
Peptide Quick Tips. Helpful guidelines for proper storage and resuspension of your peptide for optimal performance in your assay.
ChiPros Chiral Amines
Chiral amines play an important role in stereoselective organic synthesis. They are used directly as resolving agents, building blocks, or chiral auxiliaries.
Alkaline Phosphatase Protocol
Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.
Troubleshooting Strategies in GST-tagged Protein Purification
This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.
Enzymatic Deglycosylation
Enzymatic Deglycosylation Strategies: Sequential hydrolysis of individual monosaccharides from glycans can be useful for the elucidation of the structure and function of the glycan component.
Calf Intestinal (CIP) Alkaline Phosphatase for Nucleic Acid Dephosphorylation
CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.
Removal of GST Tag by Enzymatic Cleavage
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
Introduction to Target Identification with Hyas and Dione Kits
In cases where photoaffinity labeling (PAL) probes are ineffective, Hyas and Dione Kits provide an effective method for small molecule identification by using an Iridium photocatalyst tethered to the molecule under investigation.
Glycoprotein Deglycosylation
Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.
Peptidoglycan Structure and Biosynthesis
Peptidoglycan structure: alternating GlcNAc and N-acetylmuramic acid cross-linked to peptides form the basic backbone.
Phosphorylation Basics
Explore phosphorylation types converting ADP to ATP, comparing oxidative and substrate-level phosphorylation with explanatory diagrams.
Designing Peptides
Consider sequence length, solubility, and stability when designing peptides for custom synthesis.
Mass Spectrometry of Glycans
Explore mass spectrometry analysis of glycans for glycomic & glycoproteomic neutral & acidic glycan analysis. See a general mass spec glycan analysis procedure.
Phosphorylation Analysis Tools
Phosphorylation's role in cell signaling pathways and protein modification.
Y-Shape Peg Derivatives in Drug Delivery
Immune system defends against disease by hydrolyzing foreign molecules; proteases, nucleases, liver, and kidneys are involved.
Glycosyltransferases
Enzymatic glycosyltransferase specificity challenges the one enzyme-one linkage concept.
Peptide Stability
Consider sequence length, solubility, and stability when selecting peptides for custom synthesis.
Glycosyltransferases: Tools for Synthesis and Modification of Glycans
Explore tools for glycosyltransferase synthesis and modification of glycans, such as glycosyltransferases and nucleotide sugar donors.
Peptide Coupling Reagents Guide
Novabiochem® coupling reagents for in situ activation are fast-reacting and compatible with various amino acids.
Glycosylphosphatidylinisotol-anchored Glycoproteins Overview
Glycosylphosphatidylinisotol- (GPI) anchored proteins have been identified throughout a broad range of eukaryotic species ranging from humans to insects, yeasts, bacteria, and fungi, suggesting that they are an ancient modification.
Cell Proliferation EdU Assay for DNA Synthesis Detection
Explore the EdU Cell Proliferation Assay for fast DNA synthesis detection in cell biology, oncology, and drug research.
Ni-NTA-Atto Conjugates
Ni-NTA-Atto conjugates provide specific and highly sensitive detection of His-tagged fusion proteins.
ChemMatrix® Resin for solid phase peptide synthesis
ChemMatrix® is a proprietary, 100% PEG (polyethylene glycol) based resin from PCAS BioMatrix.
Page 1 of 3