Protein pulldown

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To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.

Imprint® Chromatin Immunoprecipitation Kit (CHP1) Protocol

The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format

Immunoprecipitation Kit (Protein G) Protocol & Troubleshooting

Immunoprecipitation Kit (Protein A) Protocol & Troubleshooting

Sera-Mag™ SpeedBeads Magnetic Protein A/G-Particles Protocol

Sera-Mag SpeedBeads Protein A/G Magnetic Particles provide a fast and convenient method for both manual and automated magnetic isolation of proteins using affinity binding. The particles can be used for isolating antibodies from serum, cell culture supernatant or ascites, and

Purification from Unclariﬁed Cell Lysate using HisTrap™ FF Crude

This page shows how to purify histidine-tagged recombinant proteins from unclariﬁed cell lysate using HisTrap FF crude from Cytiva.

Performing a Purification of IgG Antibodies with MabSelect Sure

This page shows how to separate IgG antibodies by afﬁnity chromatography using MabSelect Sure from Cytiva.

Performing a Separation with Cytiva Products Based on GST Fusion Proteins

How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from Cytiva.

Performing a Purification of IgG Antibodies with Protein G HP SpinTrap™/Ab SpinTrap™

This page shows how to separate IgG antibodies by afﬁnity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from Cytiva.

Antibody Purification using Protein A, Protein G, or Protein L Agarose

Antibody Purification using Protein A, Protein G, or Protein L Agarose protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should be noted that if the starting material is serum

Dextran

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(1-6) linkages. The remaining a(1-3) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three

Dextran Sulfate

Dextran sulfates are supplied as the sodium salt forms, making them soluble and stable in water. Dextran sulfate contains approximately 17% sulfur which is equivalent to approximately 2.3 sulfate groups per glucosyl residue.

Protocols for Membrane Scaffold Proteins and Nanodisc Formation

Nanodisc technology offers a means to overcome some of the challenges associated with solubilizing membrane proteins. Nanodisc technology is applicable to a wide variety of membrane proteins and protein classes.