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Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
Purification using StrepTactin Sepharose® High Performance
This page shows how to purify Strep-tag II recombinant proteins using StrepTactin Sepharose® High Performance from Cytiva.
Purification of Histidine-Tagged Proteins using TALON® Superflow
This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.
SOURCE™: Purification at High Throughput with High Resolution and Easy Scale-Up
This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.
Refolding of proteins using gel filtration
This page shows how to refold proteins using gel filtration and products from Cytiva.
Refolding: Ion Exchange Chromatography
Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.
Removal of Albumin Using Blue Sepharose® Chromatography Media
Remove albumin from affinity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from Cytiva.
Sepharose Big Beads: Purification from Crude, Viscous Samples at Large Scale
This page covers how and why to use Sepharose Big Beads for purification from crude, viscous samples.
Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up
This page covers the use of Sepharose Fast Flow for purification of proteins.
Sepharose High Performance: Purification with High Resolution
This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.
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