Purification

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Performing a Purification with Calmodulin Sepharose 4B

This page shows how to purify ATPases, adenylate cyclases, protein kinases, phosphodiesterases and neurotransmitters by affinity chromatography using Calmodulin Sepharose 4B from Cytiva.

Puriﬁcation Using MBPTrap™ HP Columns

This page shows how to purify MBP-tagged proteins using MBPTrap HP columns from Cytiva.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Tag Removal by Enzymatic Cleavage

This protocol shows how to remove histidine tags by enzymatic cleavage using Cytiva products.

Capto Adhere ImpRes

Capto adhere ImpRes is a cost-effective and ﬂexible chromatography medium from Cytiva, designed for high-resolution polishing of MAbs.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Sepharose Big Beads: Purification from Crude, Viscous Samples at Large Scale

This page covers how and why to use Sepharose Big Beads for purification from crude, viscous samples.

SOURCE™: Purification at High Throughput with High Resolution and Easy Scale-Up

This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.

Sepharose XL – for selected proteins that require very high binding capacity to increase productivity, easy scale-up

This page covers how and when to use Sepharose XL media for purification of proteins.

Column Packing and Preparation for Size Exclusion Chromatography

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

Reverse Phased Chromatography (RPC) in Practice

In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.

Refolding: Ion Exchange Chromatography

Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.

Antibody Purification using Protein A/G/L Agarose

Antibody purification protocols yield preparations containing endogenous IgG alongside specific antibodies.

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