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DNA and RNA purification
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facet applications:DNA and RNA purification
facet content type:Technical Article
Showing 1-30 of 37 results
Sodium acetate buffer solution for molecular biology
Sodium Acetate Buffer provider. 5+ decades providing high-quality buffer products that maintain consistent performance for molecular biology .
Protein Affinity Chromatography
Affinity chromatography is the process of bioselective adsorption and subsequent recovery of a compound from an immobilized ligand. This process allows for the highly specific and efficient purification of many diverse proteins and other compounds. The process requires
High Pure Technology and Silica Adsorption Kits
The High Pure and silica adsorption kits developed by Roche depend on the tendency of nucleic acids to adsorb to silica (glass) in the presence of a chaotropic salt such as sodium iodide (NaI), guanidine thiocyanate or guanidine hydrochloride [Melzak
Oligonucleotide Quality Control by Analytical HPLC
In many cases oligonucleotides require quantitative analysis to verify specifications have been met. MALDI-TOF and ESI-MS can provide qualitative-quantification for purity.
ECACC Nucleic Acids
DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.
Sera-Mag™ and Sera-Mag SpeedBeads Magnetic Particles
Sera-Mag and Sera-Mag SpeedBeads provide cost effective magnetic bead separation technology for molecular biology applications, nucleic acid isolation, and research immunoassays.
General Considerations for Plasmid DNA Preparation
This page discusses methods for plasmid DNA purification and the factors impacting resulting DNA purity and yield using illustra plasmidPrep Kits from Cytiva.
RNA Preparation Troubleshooting
This page shows possible causes and solutions for problems that may occur during RNA preparation.
Genomic DNA Preparation Troubleshooting
Description of common problems that can occur during genomic DNA preparation
DNA and RNA Quantification
An overview of methods for quantifying DNA and RNA, how to measure DNA and RNA concentration and yield, and how to assess purity of nucleic acid samples.
How Proteinase K Can Be Used in Blood DNA Extraction Applications
In blood DNA extraction, Proteinase K, an enzyme commonly used to degrade proteins, can help break down the cellular and nuclear membranes, releasing DNA from the cells that protect it from degradation and increase purity/yield making it more suitable for
An Introduction to Proteinase K and Its Applications
Proteinase K is commonly used in molecular biology and biochemistry applications to digest structural proteins and enzymes. It is useful in removing nucleases that can degrade DNA and RNA, as well as in the isolation of intact genomic DNA from
Nuclease-Free Water at Your Fingertips
An ultrafiltration cartridge can be placed at the outlet of a water purification system to deliver nuclease-free ultrapure water.
Long-term Storage of Blood in DNAgard® Blood
Two separate studies were conducted to analyze long-term storage of blood in DNAgard Blood stabilization reagent.
Stabilizing RNA at room temperature in RNAstable®
RNAstable® is a novel preservation product developed to protect
RNA from degradation during storage or shipment at ambient
temperatures. The synthetic storage medium is based on the natural principles of anhydrobiosis (meaning “life without water”), a biological mechanism employed by some organisms
Extract-N-Amp™ Plant Tissue PCR Kits
The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that
Genomic DNA Sample Purification and Quality Assessment
The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of
Biomatrica® offers an innovative alternative to reliance on cold chain logistics
Biomatrica® products enable researchers to stabilize, ship, and store biological samples for long periods of time.
RNA Isolation from Blood, Cultured Cells, and Animal or Plant Tissue on Whatman™ FTA™ Cards
This article describes the evaluation of Whatman FTA cards from Cytiva for their ability to collect, store, and isolate high-quality RNA from a variety of crude biological samples.
Downstream Applications after Genomic DNA Purification
This page shows downstream applications after genomic DNA purification.
Plasmid DNA Preparation Troubleshooting
This page shows possible causes and solutions for problems that may occur during plasmid DNA preparation.
Procedure for Purifying and Concentrating DNA using illustra™ GFX 96 PCR Purification Kit
This page shows the procedure for purifying and concentrating DNA using illustra™ GFX 96 PCR Purification Kit from Cytiva.
Oligonucleotide Array CGH Analysis of a Robust Whole Genome Amplification Method
In recent years, array-based Comparative Genomic Hybridization (aCGH) has been refined to determine chromosomal changes at progressively higher resolutions. This evolving technology is, however, somewhat hampered by the large DNA input requirement—a minimum of 150,000 copies of a human genome
Extract-N-Amp Reagent Provides a Highly Effective Way of Generating PCR Products from FTA® Blood Cards
Extract-N-Amp is a versatile, combined DNA extraction and amplification kit intended to simplify the generation of PCR products from a range of sample types. This methodology can be used without modification to perform PCR on blood samples stored on FTA
Viral RNA Extraction Buffer for SARS-CoV-2 in Saline and Saliva
Viral RNA Extraction Buffer (VRE100) and related reagents for qRT-PCR and additional viral RNA analyses.
Complete Solutions for PCR Assay Development
Fit-for-use products offer the quality, consistency & documentation necessary for every step of your IVD development and manufacturing process.
Benzonase® Nuclease for Microbiome Workflows
Benzonase® Nuclease for reducing host DNA in microbiome workflows and enhancing taxa identification.
MetaPolyzyme, DNA Free Cell Lysis Enzymes for Microbiome Workflows | Sigma-Aldrich
Explore the benefits of MetaPolyzyme, DNA free to improve DNA extraction and increase the number of taxa identified in treated samples.
How to Determine the Best Product & Supplier for Proteinase K
The cost of proteinase K can vary depending on the source, purity, manufacturing process and vendor’s quality management system. It is important to balance the cost with the desired quality, performance, documentation and technical/quality support to select the optimal Proteinase
Guide on How to Use Proteinase K in Different Procedures
Guidelines on use of proteinase K, an enzyme commonly used to degrade proteins, and protect DNA and RNA from degradation in samples.
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