general particulate removal

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Answers to common syringe filter selection questions, such as sterile vs non-sterile, how to use, membrane and housing types, and pore size.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Retentate filtration with Millicup™-FLEX disposable vacuum filtration units

Methods and tutorial video for filtration techniques in contamination analysis using a faster alternative to glass vacuum filtration systems.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

How to Extend Liquid Chromatography Column Life: Regenerate or Use Guard Columns

What is causing aging of a column? Is the regeneration/washing of the column a reasonable approach to extend the lifetime of a column? What are the risks & challenges? Learn more in this article.

Filter Paper

Whatman® filter papers for qualitative and quantitative analysis.

IgG Antibody Purification with HiTrap® Protein G HP Columns

This page shows how to separate IgG antibodies by afﬁnity chromatography using HiTrap® Protein G HP columns from Cytiva.

Performing a Separation with Sephadex^®

This page shows how to perform a separation with a Sephadex column from Cytiva which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.

Column, Media and Sample Preparation for Hydrophobic Interaction Chomatography

Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with Cytiva media are detailed, including sample application, load volume and temperature

Water for Clinical Chemistry

Protein Sample Ultrafiltration

This page discusses the principles and benefits of using ultrafiltration membranes in protein sample preparation. Possible applications include solvent exchange, protein sample concentration, or sample purification.

Solubility Guidelines for Peptides

Improper solubilization can potentially result in loss of the peptide/protein and result in failure of the experiment.

Puriﬁcation of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using Ni Sepharose® Excel

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from Cytiva.

Equipment for the Cell Culture Laboratory

General cell culture laboratory equipment including cell culture incubators, biosafety cabinets, plates, flasks, pipettes, vials, tubes and filtration units used to culture cells. Free ECACC handbook download.

cOmplete™ His-Tag Purification Resin Protocol & Troubleshooting

Tips & Tricks | Sample Handling and Preparation | MILLIPLEX^® Assays

Have questions about sample handling and preparation for MILLIPLEX® multiplex panels? Explore information on sample collection, sample handling, and sample preparation for multiplex assays with tips and tricks to get the most out of your assays.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Nucleic Acid Sample Preparation Methodology

Spectrophotometry can be used to estimate DNA or RNA concentration and to analyze the purity of the preparation.

cOmplete™ His-Tag Purification Column Protocol & Troubleshooting

Purification Protocols: cOmplete His-Tag Purification Columns are compatible with automated chromatography systems such as ÄKTAexplorer.

Electrochemistry on the Bench and in the Field

Nanomaterials, electrochemistry, BASi, LIB, Lithium Ion Batteries, reference electrode, Teflon, silver nitrate, working electrodes, counter electrode, organic electrosynthesis

Solubility enhancement of hydrophobic drugs via drug-loaded micelles using biodegradable PEG- polyester diblock copolymers

One of the common difficulties with intravenous drug delivery is low solubility of the drug. The requirement for large quantities of saline to dissolve such materials limits their clinical use, and one solution for this problem that has recently generated

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Reactive Silver Inks for High-Performance Printed Electronics

The ability to pattern conductive electrodes is technologically relevant for several applications, including photovolatics, displays, sensors, and biomedical devices.

Chromatofocusing: Principles and Methods

This page covers the principles and methods of chromatofocusing, a chromatography technique that separates proteins according to differences in their isoelectric point (pI).

Correlation of Angiogenesis Biomarkers with NSCLC

The angiogenesis pathway is complex with many targets to analyze. Simultaneous quantification of multiple markers, with the MILLIPLEX® Human Angiogenesis/Growth Factor Panel 1, saves valuable resources. See how this panel was used to correlate angiogenesis biomarkers with early metastatic progression