HT-1080 cells were plated in a chamber slide and transduced with LentiBrite™ GFP-LC3 lentiviral particles at an MOI of 20 for 24 hours. After media replacement and 48 hours further incubation, cells were incubated for 4 hours in EBSS containing
Cell based autophagy assays including live cell LC3 GFP/RFP lentiviral biosensors, kits for autophagy detection and autophagy activators and inhibitors.