quality analysis, we offer
a wide range of high purity mobile phase additives for
LC-MS applications. The LC-MS portfolio includes the
most commonly used acids, bases and volatile salts of
high purity
www.sigma-aldrich.com/lcms-solvents
LC-MS
Solvents
LC-MS
Reagents
Discover LC-MS reagents
LC-MS Reagents
We offer LiChropur® products specific for LC-MS analysis.
These reagents are tested for
HPLC
Proteomix® SCX-NP5 (2.1 mm x 50) mm, 5 μm Z777156
Acetonitrile, LiChrosolv® hypergrade for LC-MS 1.00029
Water, LiChrosolv® for LC-MS 1.15333
Ammonium acetate, LiChropur™ for LC-MS
533, and
EPA 8327 was completed in 5 minutes in this application note.
The HPLC column of choice for PFAS analysis by LC-MS/(MS) is a C18
column based on fully porous silica particles (FPP) or on superficially
additive for LC-MS,
LiChropur™, ≥99.0%
70221
Formic acid, for HPLC LiChropur™ 5.43804
Acetonitrile, gradient grade LiChrosolv® Reag. Ph Eur 1.00030
Find more information on Cannabis analysis at
LC-ESI-MS-MS. High purity water was utilized and results highlight the
importance of low organic contamination to reach low levels of detection.
Denaturating HPLC is a reverse phase ion pairing HPLC method
2.14
Figure 36
LC-MS (top) and LC-MS/MS (bottom) analysis of internal standard D3-methyl malonic acid (left) and methylmalonic acid (right) in human plasma.
For the LC-MS/MS quantification experiments
hypergrade for LC-MS LiChrosolv® 1.06035
Water for chromatography (LC-MS grade) LiChrosolv® or ultrapure water from a Milli-Q® IQ 7 series water purification system 1.15333
Ammonium acetate LiChropur™, eluent
Ascentis® Express Biphenyl Column
High resolution in Food & Beverage
HPLC Analysis of Maté Leaves using an Ascentis® Express C18 Column
LC-MS/MS Analysis of Multiple Mycotoxins using an Ascentis
for MS 900682
Acetonitrile, for UHPLC, suitable for MS 900667
Guanidine hydrochloride, ≥99% (titration), organic base and chaeotropic agent G4505
Difluoroacetic acid, for LC-MS, LiChropur™ 00922
environmental waters, as well in some tap waters.
• The high purity water used in analytical laboratories, be it bottled HPLC-grade, LC-MS grade, or from a water purification system, comes from tap
(Unknown identification by LC-MS)
Trace Process Impurity Identification and Quantitation (Low concentration and/or related to degradation
study analysis)
Critical LC-MS Impurity Assay
Toxicology
350 µL of water. LC-MS/MS analysis was performed
using a Shimadzu 8030 triple quad mass spectrometer.
Chromatographic separation was performed using
Ascentis® Express Phenyl-Hexyl HPLC column (2.7 μm;
Water, LiChrosolv® for LC-MS 1153334000 Supelco®
Formic acid, LiChropur™ for LC-MS 533002 Supelco®
Accessories
Autosampler vials volume 0.3 mL 29661-U Supelco®
To place an order or receive
washed with 0.1% formic acid in isopropanol at 0.2 mL/min for 48 min.
Overview of U/HPLC columns for LC-MS
For LC-MS, particulate HPLC columns, preferably based
on high purity Type B silica, are widely
achieve both a high level of LC-MS instrumentation
sensitivity as well as a reliable and traceable analytical process.
For more information about small molecule bioanalysis by LC-MS and LC-MS/MS, please visit
cloudiness which can
interfere with the UV spectroscopic analysis. If a
precipitate is found, other analytical methods, such as HPLC
or LC/MS/MS, may be used. Some compounds may contain
color-producing
(heptane sulfonic acid, octane sulfonic acid) and used for bases; and where quaternary amines
(tetrabutylammonium chloride) are used for acids.
Choose the right HPLC Column
Chromatographic
run prior to analysis. Purospher® STAR RP-18 end-
capped HPLC columns give ideally low and very stable
background signals in LC/MS, simply after washing
with 2-propanol / 0.1% formic acid.
0.0
0.2
characterized by NMR, HPLC
and MS, high purity (95%)
• Custom synthesis of standards offered
• Compatible with labeling by reductive amination,
permethylation, and sialic acid derivatization.
characterized by NMR, HPLC,
and MS, high purity (95%)
• Custom synthesis of standards offered
• Compatible with labeling by reductive amination,
permethylation, and sialic acid derivatization