www.sigma-aldrich.com/lcms-solvents
LC-MS
Solvents
LC-MS
Reagents
Discover LC-MS reagents
LC-MS Reagents
We offer LiChropur® products specific for LC-MS analysis.
These reagents are tested for
quality analysis, we offer
a wide range of high purity mobile phase additives for
LC-MS applications. The LC-MS portfolio includes the
most commonly used acids, bases and volatile salts of
high purity
HPLC
Proteomix® SCX-NP5 (2.1 mm x 50) mm, 5 μm Z777156
Acetonitrile, LiChrosolv® hypergrade for LC-MS 1.00029
Water, LiChrosolv® for LC-MS 1.15333
Ammonium acetate, LiChropur™ for LC-MS
533, and
EPA 8327 was completed in 5 minutes in this application note.
The HPLC column of choice for PFAS analysis by LC-MS/(MS) is a C18
column based on fully porous silica particles (FPP) or on superficially
additive for LC-MS,
LiChropur™, ≥99.0%
70221
Formic acid, for HPLC LiChropur™ 5.43804
Acetonitrile, gradient grade LiChrosolv® Reag. Ph Eur 1.00030
Find more information on Cannabis analysis at
intervals of analytical instruments.
A minimization can be achieved by using high-purity LC-MS specified solvents. LC-MS grade solvents from
Merck Millipore meet all the requirements of the user concerning
selection of salts and organic solvents used in mobile phase preparation, but selecting the best type of water is sometimes an arduous task.
• LC-MS/MS requires the use of high purity water to prepare
min.
Overview of U/HPLC columns for LC-MS
For LC-MS, particulate HPLC columns, preferably based
on high purity Type B silica, are widely used.
1. Small particles deliver high separation efficiency
Photo-oxidation
Conclusion
Optimized HPLC analyses require the use of high purity solvents and reagents. While chromatographers take great
care in the selection of salts and organic solvents used in mobile
350 µL of water. LC-MS/MS analysis was performed
using a Shimadzu 8030 triple quad mass spectrometer.
Chromatographic separation was performed using
Ascentis® Express Phenyl-Hexyl HPLC column (2.7 μm;
hypergrade for LC-MS LiChrosolv® 1.06035
Water for chromatography (LC-MS grade) LiChrosolv® or ultrapure water from a Milli-Q® IQ 7 series water purification system 1.15333
Ammonium acetate LiChropur™, eluent
chromatographers take great care to assure the purity of salts, organic solvents, and
other HPLC mobile phase components, they often take water quality for granted. High-purity water comprises by far the largest
® before UV treatment
HPLC grade bottled water
Milli-Q® water at the point of use
Figure 1. Chromatograms at 210 nm of
four different types of water
Figure 2. LC-MS chromatograms (ESI +) of
Acetonitril, Ref.
HPLC-Purity
Stability of the TC-Group in SPOS Reagents
CapA/B
• Treatment of Bz-rG(ib)tc-Bz model compound with CapA/B mix
for 20 h at 25 ºC
• Analysis via LC-MS
20 h: 3.0% 95.4%
LC-ESI-MS-MS. High purity water was utilized and results highlight the
importance of low organic contamination to reach low levels of detection.
Denaturating HPLC is a reverse phase ion pairing HPLC method
(Unknown identification by LC-MS)
Trace Process Impurity Identification and Quantitation (Low concentration and/or related to degradation
study analysis)
Critical LC-MS Impurity Assay
Toxicology
to
ensure high application security
• OmniSolv® solvents: offered in different grades for GC, high resolution GC (HR-GC),
purge and trap, gradient HPLC, and LC/MS; outstanding purity and batch-to-batch
Fill 1 ml from the upper phase into a vial
LC-MS/MS analysis (use an appropriate UHPLC system)
MS-MS detector: Q Trap MS/MS system or similar
HPLC column: Fused core C18 (10 cm x 3,0 mm, 2,7μm)
Fill 1 ml from the upper phase into a vial
LC-MS/MS analysis (use an appropriate UHPLC system)
MS-MS detector: Q Trap MS/MS system or similar
HPLC column: Fused core C18 (10 cm x 3,0 mm, 2,7μm)
siRNA oligos - ESI/MS
antiGAPDH siRNA
AntiGAPDH siRNA were synthesized; deprotected using neat EDA, and purified using
RP-HPLC. Identity of oligonucleotides confirmed by LC/ESI-MS
siRNA Sequence
molecules when working with MS detection.
Solvent and additive purity
The quality of the solvents as well as of the additives
(buffers, salts, acids, bases) utilized in an HPLC
experiment has to be adapted
inlet and the outlet of the cartridge.
LC-MS
The LC-Pak™ polisher is designed for sensitive analytical methods such as LC-MS or
UHPLC-MS. High purity water containing traces of organic molecules
Buffers for LC-MS
should be prepared using the purest salt and acid/
base quality available. If possible, avoid working
with an ammonium bicarbonate buffer. The salt is
often highly contaminated
achieve both a high level of LC-MS instrumentation
sensitivity as well as a reliable and traceable analytical process.
For more information about small molecule bioanalysis by LC-MS and LC-MS/MS, please visit