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Showing 1-30 of 54 results for "M1882" within Papers
Computer-controlled perifusion system for neuroendocrine tissues: development and applications.
A Negro-Vilar et al.
Methods in enzymology, 124, 67-79 (1986-01-01)
Raja Ghosh
Journal of chromatography. A, 1468, 164-172 (2016-10-01)
Columns with relatively short bed-height to diameter ratios are frequently used for process-scale chromatography applications such as biopharmaceutical purification. Non-uniform flow distribution within such columns could result in broad and poorly resolved eluted peaks, which could in turn affect purity
Lorena Fuentes-Broto et al.
Antioxidants (Basel, Switzerland), 10(11) (2021-11-28)
Retinitis pigmentosa (RP) is a group of inherited neurodegenerative diseases characterized by a progressive loss of visual function that primarily affect photoreceptors, resulting in the complete disorganization and remodeling of the retina. Progression of the disease is enhanced by increased
Lorena Perdices et al.
Antioxidants (Basel, Switzerland), 9(8) (2020-08-14)
Retinitis pigmentosa (RP) includes a group of genetic disorders that involve the loss of visual function due to mutations mainly in photoreceptors but also in other retinal cells. Apoptosis, retinal disorganization, and inflammation are common in the progression of the
Keith A Oberg et al.
Protein science : a publication of the Protein Society, 12(9), 2015-2031 (2003-08-22)
Protein basis sets have been extensively used as reference data for the determination of protein structure with optical methods such as circular dichroism and infrared spectroscopies. We have taken a new approach to basis protein selection by utilizing three crystal
Guadalupe Gómez-Baena et al.
Scientific reports, 9(1), 10757-10757 (2019-07-26)
Major urinary proteins (MUP) are the major component of the urinary protein fraction in house mice (Mus spp.) and rats (Rattus spp.). The structure, polymorphism and functions of these lipocalins have been well described in the western European house mouse
H Jansson et al.
The Journal of chemical physics, 130(20), 205101-205101 (2009-06-03)
In this quasielastic neutron scattering (QENS) study we have investigated the relation between protein and solvent dynamics. Myoglobin in different water:glycerol mixtures has been studied in the temperature range of 260-320 K. In order to distinguish between solvent and protein
Brandye M Smith et al.
Analytical chemistry, 74(16), 4076-4080 (2002-08-30)
The application of single-pass attenuated total reflection Fourier transform infrared (ATR-FT-IR) microscopy was investigated for secondary structure analysis of 15 representative proteins in H2O solution. This is the first reported application of single-pass ATR-FT-IR for protein analysis; thus, the method
Kara A Zielinski et al.
IUCrJ, 10(Pt 3), 363-375 (2023-05-05)
Advances in time-resolved structural techniques, mainly in macromolecular crystallography and small-angle X-ray scattering (SAXS), allow for a detailed view of the dynamics of biological macromolecules and reactions between binding partners. Of particular promise, are mix-and-inject techniques, which offer a wide
V Kery et al.
The Journal of biological chemistry, 269(41), 25283-25288 (1994-10-14)
The first committed step of transsulfuration is catalyzed by cystathionine beta-synthase (CBS), a known pyridoxal 5'-phosphate (PLP) enzyme. The inferred amino acid sequences of rat liver CBS and rat liver hemoprotein H-450 are identical. We now confirm the presence of
Stephanie Moore et al.
Journal of chromatography. A, 1476, 1-8 (2016-11-24)
Despite the developments for faster liquid chromatographic and mass spectral detection techniques, the standard in-solution protein digestion for proteomic analyses has remained relatively unchanged. The typical in-solution trypsin protein digestion is usually the slowest part of the workflow, albeit one
A Leber et al.
The Journal of biological chemistry, 274(53), 37658-37664 (1999-12-23)
Human endothelial nitric-oxide synthase (eNOS) was expressed in the methylotrophic yeast Pichia pastoris, making use of the highly inducible alcohol oxidase promoter. The recombinant protein constituted approximately 3% of total protein and was largely soluble (>75%). About 1 mg of
Matthew B Lohse et al.
Genetics, 216(2), 409-429 (2020-08-26)
An unusual feature of the opportunistic pathogen Candida albicans is its ability to switch stochastically between two distinct, heritable cell types called white and opaque. Here, we show that only opaque cells, in response to environmental signals, massively upregulate a
Cassandra M Stawicki et al.
Scientific reports, 11(1), 19921-19921 (2021-10-09)
Fluorescently labeled antibody and aptamer probes are used in biological studies to characterize binding interactions, measure concentrations of analytes, and sort cells. Fluorescent nanoparticle labels offer an excellent alternative to standard fluorescent labeling strategies due to their enhanced brightness, stability
Peng-Fei Guo et al.
Nanoscale, 10(26), 12535-12542 (2018-06-23)
A novel organic-inorganic hybrid was prepared by anchoring (3-aminopropyl)triethoxysilane (APTES) on the surface of monolayer titanate nanosheets and subsequent modification with hydrophilic polyethylene glycol (PEG). The PEGylated hydrophilic monolayer titanate nanosheets were abbreviated as PEG-APTES-TiNSs, and they exhibit a lateral
Peng-Fei Guo et al.
Nanoscale, 11(19), 9362-9368 (2019-05-01)
Boron-titanate monolayer nanosheets were prepared through a scalable step by step intercalation approach for anchoring 3-mercaptopropyltriethoxysilane (MPTS) on the surface. MPTS provides clickable sites with 4-vinylphenylboronic acid (VPBA) via a thiol-ene (TE) click reaction to obtain monolayer titanate nanosheets with
Ying Guo et al.
BMC microbiology, 8, 226-226 (2008-12-19)
Iron is recognized as an important trace element, essential for most organisms including pathogenic bacteria. HugZ, a protein related to heme iron utilization, is involved in bacterial acquisition of iron from the host. We previously observed that a hugZ homologue
Joseph Gault et al.
Nature methods, 17(5), 505-508 (2020-05-07)
Ligands bound to protein assemblies provide critical information for function, yet are often difficult to capture and define. Here we develop a top-down method, 'nativeomics', unifying 'omics' (lipidomics, proteomics, metabolomics) analysis with native mass spectrometry to identify ligands bound to
Role of bound zinc in dimer stabilization but not enzyme activity of neuronal nitric-oxide synthase.
B Hemmens et al.
The Journal of biological chemistry, 275(46), 35786-35791 (2000-08-24)
Nitric-oxide synthases (NOS) are homodimeric proteins and can form an intersubunit Zn(4S) cluster. We have measured zinc bound to NOS purified from pig brain (0.6 mol/mol of NOS) and baculovirus-expressed rat neuronal NOS (nNOS) (0.49 +/- 0.13 mol/mol of NOS)
Hajime Shigemitsu et al.
Nature communications, 11(1), 3859-3859 (2020-08-02)
Non-enzymatic proteins including antibodies function as biomarkers and are used as biopharmaceuticals in several diseases. Protein-responsive soft materials capable of the controlled release of drugs and proteins have potential for use in next-generation diagnosis and therapies. Here, we describe a
Lavanya Tandon et al.
International journal of biological macromolecules, 156, 576-584 (2020-04-12)
Fluorescence studies were performed to determine the photophysical behavior of heme group in the presence of cationic Gemini surfactants of different architectures. Both hemoglobin and myoglobin were used to understand the heme group interactions with Gemini surfactants under the influence
Cevahir Altinkaynak et al.
Chemistry & biodiversity, 20(6), e202201136-e202201136 (2023-05-02)
Protein-inorganic hybrid nanoflowers are new multifunctional materials shown enhanced catalytic performance. Specially, they are used as catalyst and dye decolorizer via Fenton reaction. In this study, the Myoglobin-Zn (II) assisted hybrid nanoflowers (MbNFs@Zn) were fabricated by using myoglobin and zinc
Angelika Jochim et al.
eLife, 9 (2020-06-10)
Energy-coupling factor type transporters (ECF) represent trace nutrient acquisition systems. Substrate binding components of ECF-transporters are membrane proteins with extraordinary affinity, allowing them to scavenge trace amounts of ligand. A number of molecules have been described as substrates of ECF-transporters
Kevin Pfeifer et al.
Nanomaterials (Basel, Switzerland), 12(14) (2022-07-28)
The outermost component of cell envelopes of most bacteria and almost all archaea comprise a protein lattice, which is termed Surface (S-)layer. The S-layer lattice constitutes a highly porous structure with regularly arranged pores in the nm-range. Some archaea thrive
Jiangnan Zheng et al.
Journal of chromatography. A, 1358, 29-38 (2014-07-22)
Selective enrichment of glycopeptides from complex biological samples is essential for MS-based glycoproteomics, but challenges still remain. In this work, glucose-functionalized magnetic mesoporous nanoparticles (MMNs), which hold the attractive features of well-defined core/shell structure, high specific surface area (324m(2)/g), narrow
Marta L Mendes et al.
Molecular systems biology, 15(9), e8994-e8994 (2019-09-27)
We present a concise workflow to enhance the mass spectrometric detection of crosslinked peptides by introducing sequential digestion and the crosslink identification software xiSEARCH. Sequential digestion enhances peptide detection by selective shortening of long tryptic peptides. We demonstrate our simple
Ursula Waack et al.
mBio, 9(6) (2018-12-20)
Antibiotic-resistant Acinetobacter baumannii is increasingly recognized as a cause of difficult-to-treat nosocomial infections, including pneumonia, wound infections, and bacteremia. Previous studies have demonstrated that the metalloprotease CpaA contributes to virulence and prolongs clotting time when added to human plasma as
Meng-Meng Wang et al.
Talanta, 171, 173-178 (2017-05-30)
By virtue of the flexible structure of polyoxometalates, Ni
Petr Pompach et al.
Clinical chemistry, 62(1), 270-278 (2015-10-21)
Recent studies show that the haptoglobin phenotype in individuals with diabetes mellitus is an important factor for predicting the risk of myocardial infarction, cardiovascular death, and stroke. Current methods for haptoglobin phenotyping include PCR and gel electrophoresis. A need exists
Amandeep Kaur et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 174, 236-244 (2016-12-07)
The energetics and the impact on the conformation of heme containing protein myoglobin (Mb) due to the binding of three transition metal ions (Zn
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