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Showing 1-30 of 54 results for "M1882" within Papers
V Kery et al.
The Journal of biological chemistry, 269(41), 25283-25288 (1994-10-14)
The first committed step of transsulfuration is catalyzed by cystathionine beta-synthase (CBS), a known pyridoxal 5'-phosphate (PLP) enzyme. The inferred amino acid sequences of rat liver CBS and rat liver hemoprotein H-450 are identical. We now confirm the presence of
Lorena Fuentes-Broto et al.
Antioxidants (Basel, Switzerland), 10(11) (2021-11-28)
Retinitis pigmentosa (RP) is a group of inherited neurodegenerative diseases characterized by a progressive loss of visual function that primarily affect photoreceptors, resulting in the complete disorganization and remodeling of the retina. Progression of the disease is enhanced by increased
Raja Ghosh
Journal of chromatography. A, 1468, 164-172 (2016-10-01)
Columns with relatively short bed-height to diameter ratios are frequently used for process-scale chromatography applications such as biopharmaceutical purification. Non-uniform flow distribution within such columns could result in broad and poorly resolved eluted peaks, which could in turn affect purity
Kara A Zielinski et al.
IUCrJ, 10(Pt 3), 363-375 (2023-05-05)
Advances in time-resolved structural techniques, mainly in macromolecular crystallography and small-angle X-ray scattering (SAXS), allow for a detailed view of the dynamics of biological macromolecules and reactions between binding partners. Of particular promise, are mix-and-inject techniques, which offer a wide
Stephanie Moore et al.
Journal of chromatography. A, 1476, 1-8 (2016-11-24)
Despite the developments for faster liquid chromatographic and mass spectral detection techniques, the standard in-solution protein digestion for proteomic analyses has remained relatively unchanged. The typical in-solution trypsin protein digestion is usually the slowest part of the workflow, albeit one
Lavanya Tandon et al.
International journal of biological macromolecules, 156, 576-584 (2020-04-12)
Fluorescence studies were performed to determine the photophysical behavior of heme group in the presence of cationic Gemini surfactants of different architectures. Both hemoglobin and myoglobin were used to understand the heme group interactions with Gemini surfactants under the influence
Cevahir Altinkaynak et al.
Chemistry & biodiversity, 20(6), e202201136-e202201136 (2023-05-02)
Protein-inorganic hybrid nanoflowers are new multifunctional materials shown enhanced catalytic performance. Specially, they are used as catalyst and dye decolorizer via Fenton reaction. In this study, the Myoglobin-Zn (II) assisted hybrid nanoflowers (MbNFs@Zn) were fabricated by using myoglobin and zinc
Role of bound zinc in dimer stabilization but not enzyme activity of neuronal nitric-oxide synthase.
B Hemmens et al.
The Journal of biological chemistry, 275(46), 35786-35791 (2000-08-24)
Nitric-oxide synthases (NOS) are homodimeric proteins and can form an intersubunit Zn(4S) cluster. We have measured zinc bound to NOS purified from pig brain (0.6 mol/mol of NOS) and baculovirus-expressed rat neuronal NOS (nNOS) (0.49 +/- 0.13 mol/mol of NOS)
H Jansson et al.
The Journal of chemical physics, 130(20), 205101-205101 (2009-06-03)
In this quasielastic neutron scattering (QENS) study we have investigated the relation between protein and solvent dynamics. Myoglobin in different water:glycerol mixtures has been studied in the temperature range of 260-320 K. In order to distinguish between solvent and protein
Keith A Oberg et al.
Protein science : a publication of the Protein Society, 12(9), 2015-2031 (2003-08-22)
Protein basis sets have been extensively used as reference data for the determination of protein structure with optical methods such as circular dichroism and infrared spectroscopies. We have taken a new approach to basis protein selection by utilizing three crystal
Guadalupe Gómez-Baena et al.
Scientific reports, 9(1), 10757-10757 (2019-07-26)
Major urinary proteins (MUP) are the major component of the urinary protein fraction in house mice (Mus spp.) and rats (Rattus spp.). The structure, polymorphism and functions of these lipocalins have been well described in the western European house mouse
Brandye M Smith et al.
Analytical chemistry, 74(16), 4076-4080 (2002-08-30)
The application of single-pass attenuated total reflection Fourier transform infrared (ATR-FT-IR) microscopy was investigated for secondary structure analysis of 15 representative proteins in H2O solution. This is the first reported application of single-pass ATR-FT-IR for protein analysis; thus, the method
Hajime Shigemitsu et al.
Nature communications, 11(1), 3859-3859 (2020-08-02)
Non-enzymatic proteins including antibodies function as biomarkers and are used as biopharmaceuticals in several diseases. Protein-responsive soft materials capable of the controlled release of drugs and proteins have potential for use in next-generation diagnosis and therapies. Here, we describe a
Peng-Fei Guo et al.
Nanoscale, 10(26), 12535-12542 (2018-06-23)
A novel organic-inorganic hybrid was prepared by anchoring (3-aminopropyl)triethoxysilane (APTES) on the surface of monolayer titanate nanosheets and subsequent modification with hydrophilic polyethylene glycol (PEG). The PEGylated hydrophilic monolayer titanate nanosheets were abbreviated as PEG-APTES-TiNSs, and they exhibit a lateral
Joseph Gault et al.
Nature methods, 17(5), 505-508 (2020-05-07)
Ligands bound to protein assemblies provide critical information for function, yet are often difficult to capture and define. Here we develop a top-down method, 'nativeomics', unifying 'omics' (lipidomics, proteomics, metabolomics) analysis with native mass spectrometry to identify ligands bound to
Ying Guo et al.
BMC microbiology, 8, 226-226 (2008-12-19)
Iron is recognized as an important trace element, essential for most organisms including pathogenic bacteria. HugZ, a protein related to heme iron utilization, is involved in bacterial acquisition of iron from the host. We previously observed that a hugZ homologue
Matthew B Lohse et al.
Genetics, 216(2), 409-429 (2020-08-26)
An unusual feature of the opportunistic pathogen Candida albicans is its ability to switch stochastically between two distinct, heritable cell types called white and opaque. Here, we show that only opaque cells, in response to environmental signals, massively upregulate a
Lorena Perdices et al.
Antioxidants (Basel, Switzerland), 9(8) (2020-08-14)
Retinitis pigmentosa (RP) includes a group of genetic disorders that involve the loss of visual function due to mutations mainly in photoreceptors but also in other retinal cells. Apoptosis, retinal disorganization, and inflammation are common in the progression of the
Computer-controlled perifusion system for neuroendocrine tissues: development and applications.
A Negro-Vilar et al.
Methods in enzymology, 124, 67-79 (1986-01-01)
Cassandra M Stawicki et al.
Scientific reports, 11(1), 19921-19921 (2021-10-09)
Fluorescently labeled antibody and aptamer probes are used in biological studies to characterize binding interactions, measure concentrations of analytes, and sort cells. Fluorescent nanoparticle labels offer an excellent alternative to standard fluorescent labeling strategies due to their enhanced brightness, stability
A Leber et al.
The Journal of biological chemistry, 274(53), 37658-37664 (1999-12-23)
Human endothelial nitric-oxide synthase (eNOS) was expressed in the methylotrophic yeast Pichia pastoris, making use of the highly inducible alcohol oxidase promoter. The recombinant protein constituted approximately 3% of total protein and was largely soluble (>75%). About 1 mg of
Angelika Jochim et al.
eLife, 9 (2020-06-10)
Energy-coupling factor type transporters (ECF) represent trace nutrient acquisition systems. Substrate binding components of ECF-transporters are membrane proteins with extraordinary affinity, allowing them to scavenge trace amounts of ligand. A number of molecules have been described as substrates of ECF-transporters
Peng-Fei Guo et al.
Nanoscale, 11(19), 9362-9368 (2019-05-01)
Boron-titanate monolayer nanosheets were prepared through a scalable step by step intercalation approach for anchoring 3-mercaptopropyltriethoxysilane (MPTS) on the surface. MPTS provides clickable sites with 4-vinylphenylboronic acid (VPBA) via a thiol-ene (TE) click reaction to obtain monolayer titanate nanosheets with
Daniele Sanna et al.
Dalton transactions (Cambridge, England : 2003), 50(44), 16326-16335 (2021-11-05)
The equilibria in the solution of three different oxidovanadium(IV) complexes, VO(dhp)2 (dhp = 1,2-dimethyl-3-hydroxy-4(1H)-pyridinonato), VO(ma)2 (ma = maltolato) and VO(pic)2(H2O) (pic = picolinato), were examined in the temperature range of 120-352 K through a combination of instrumental (EPR spectroscopy) and
Sasha De Henau et al.
Nature communications, 6, 8782-8782 (2015-12-02)
Moderate levels of reactive oxygen species (ROS) are now recognized as redox signalling molecules. However, thus far, only mitochondria and NADPH oxidases have been identified as cellular sources of ROS in signalling. Here we identify a globin (GLB-12) that produces
Austen Terwilliger et al.
Journal of bacteriology, 197(14), 2400-2411 (2015-05-13)
Bacteria sustain an infection by acquiring nutrients from the host to support replication. The host sequesters these nutrients as a growth-restricting strategy, a concept termed "nutritional immunity." Historically, the study of nutritional immunity has centered on iron uptake because many
Gaëtan Boléa et al.
Food & function, 10(7), 3942-3954 (2019-06-15)
Lipid oxidation takes place in the gastric tract after the ingestion of a Western diet rich in ω-6 polyunsaturated fatty acids (PUFA) and red meat (heme iron). The incorporation of oxidation products such as 4-hydroxy-2-nonenal (4-HNE) into low-density lipoproteins is
M S Silva et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1104, 262-268 (2018-12-24)
Zinc (Zn) is an element essential to all living organisms and it has an important role as a cofactor of several enzymes. In fish, Zn deficiency has been associated with impaired growth, cataracts, skeletal abnormalities and reduced activity of various
D H Robertson et al.
Rapid communications in mass spectrometry : RCM, 11(7), 786-790 (1997-01-01)
Major urinary proteins (MUPs) from the urine of individual wild mice were characterized using electrospray ionization mass spectrometry (ESI-MS) and compared to MUPs from the urine of inbred mice. The wild mice showed considerable variation between individuals in the expression
Wilfred R Hagen
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 27(4-5), 497-507 (2022-07-09)
An EPR spectrometer has been developed that can be tuned to many frequencies in the range of ca 0.1-15 GHz. Applicability has been tested on ferrimyoglobin fluoride (MbF) and ferrimyoglobin cyanide (MbCN). MbF has a high-spin (S = 5/2) spectrum with 19F superhyperfine
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