MBD0049

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Showing 1-23 of 23 results for "MBD0049" within Papers

Oligonucleotide probe for the visualization of Escherichia coli/Escherichia fergusonii cells by in situ hybridization: specificity and potential applications.

B Regnault et al.

Research in microbiology, 151(7), 521-533 (2000-10-19)

There are several occasions when enumeration of Escherichia coli cells is needed. These include examination of urine specimens and water or food samples. Present methods rely on growth in more or less selective media (colony-forming units on agar or the

Klebsiella pneumoniae capsule expression is necessary for colonization of large intestines of streptomycin-treated mice.

S Favre-Bonté et al.

Infection and immunity, 67(11), 6152-6156 (1999-10-26)

The role of the Klebsiella pneumoniae capsular polysaccharide (K antigen) during colonization of the mouse large intestine was assessed with wild-type K. pneumoniae LM21 and its isogenic capsule-defective mutant. When bacterial strains were fed alone to mice, the capsulated bacteria

Spatial structure in the "Plastisphere": Molecular resources for imaging microscopic communities on plastic marine debris.

Cathleen Schlundt et al.

Molecular ecology resources, 20(3), 620-634 (2019-11-30)

Plastic marine debris (PMD) affects spatial scales of life from microbes to whales. However, understanding interactions between plastic and microbes in the "Plastisphere"-the thin layer of life on the surface of PMD-has been technology-limited. Research into microbe-microbe and microbe-substrate interactions

Fluorescence in situ hybridization in plants: recent developments and future applications.

Jiming Jiang

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 27(3), 153-165 (2019-03-11)

Fluorescence in situ hybridization (FISH) was developed more than 30 years ago and has been the most paradigm-changing technique in cytogenetic research. FISH has been used to answer questions related to structure, mutation, and evolution of not only individual chromosomes but

In vivo detection of Escherichia coli type 1 fimbrial expression and phase variation during experimental urinary tract infection.

C Struve et al.

Microbiology (Reading, England), 145 ( Pt 10), 2683-2690 (1999-10-28)

Adhesion mediated by fimbriae is thought to play an important role in the pathogenesis of urinary tract infections (UTI) by Escherichia coli. The majority of clinical isolates of E. coli from UTI are able to express type 1 fimbriae. However

Accelerated identification of Staphylococcus aureus from blood cultures by a modified fluorescence in situ hybridization procedure.

Sven Poppert et al.

Journal of medical microbiology, 59(Pt 1), 65-68 (2009-10-03)

This study evaluated fluorescence in situ hybridization (FISH) for rapid identification of Staphylococcus aureus and coagulase-negative staphylococci (CoNS) directly from blood cultures. Initially, 360 blood cultures containing Gram-positive cocci were investigated by a previously described microwave-FISH procedure: 44/49 (89.8 %)

Longitudinal investigation of the faecal microbiota of healthy full-term infants using fluorescence in situ hybridization and denaturing gradient gel electrophoresis.

Laure C Roger et al.

Microbiology (Reading, England), 156(Pt 11), 3317-3328 (2010-09-11)

From birth onwards, the gastrointestinal (GI) tract of infants progressively acquires a complex range of micro-organisms. It is thought that by 2 years of age the GI microbial population has stabilized. Within the developmental period of the infant GI microbiota

An improved protocol for quantification of freshwater Actinobacteria by fluorescence in situ hybridization.

Raju Sekar et al.

Applied and environmental microbiology, 69(5), 2928-2935 (2003-05-07)

We tested a previously described protocol for fluorescence in situ hybridization of marine bacterioplankton with horseradish peroxidase-labeled rRNA-targeted oligonucleotide probes and catalyzed reporter deposition (CARD-FISH) in plankton samples from different lakes. The fraction of Bacteria detected by CARD-FISH was significantly

Glycolytic and gluconeogenic growth of Escherichia coli O157:H7 (EDL933) and E. coli K-12 (MG1655) in the mouse intestine.

Regina L Miranda et al.

Infection and immunity, 72(3), 1666-1676 (2004-02-24)

Escherichia coli EDL933, an O157:H7 strain, is known to colonize the streptomycin-treated CD-1 mouse intestine by growing in intestinal mucus (E. A. Wadolkowski, J. A. Burris, and A. D. O'Brien, Infect. Immun. 58:2438-2445, 1990), but what nutrients and metabolic pathways

Microbiota profile in feces of breast- and formula-fed newborns by using fluorescence in situ hybridization (FISH).

Eugenia Bezirtzoglou et al.

Anaerobe, 17(6), 478-482 (2011-04-19)

The development of the gut is controlled and modulated by different interacting mechanisms such as, genetic endowment, intrinsic biological regulatory functions, environment influences and last but no least, the diet influence. Considered together with other endogenous and exogenous factors the

Order and disorder during Escherichia coli divergence.

Heather Hendrickson

PLoS genetics, 5(1), e1000335-e1000335 (2009-01-24)

Potential role of intratumor bacteria in mediating tumor resistance to the chemotherapeutic drug gemcitabine.

Leore T Geller et al.

Science (New York, N.Y.), 357(6356), 1156-1160 (2017-09-16)

Growing evidence suggests that microbes can influence the efficacy of cancer therapies. By studying colon cancer models, we found that bacteria can metabolize the chemotherapeutic drug gemcitabine (2',2'-difluorodeoxycytidine) into its inactive form, 2',2'-difluorodeoxyuridine. Metabolism was dependent on the expression of

Lupin kernel fiber consumption modifies fecal microbiota in healthy men as determined by rRNA gene fluorescent in situ hybridization.

Stuart C Smith et al.

European journal of nutrition, 45(6), 335-341 (2006-06-10)

Changes in the composition of gastrointestinal microbiota by dietary interventions using pro- and prebiotics provide opportunity for improving health and preventing disease. However, the capacity of lupin kernel fiber (LKFibre), a novel legume-derived food ingredient, to act as a prebiotic

Fluorescence in situ hybridization, a complementary molecular tool for the clinical diagnosis of infectious diseases by intracellular and fastidious bacteria.

Elsa Prudent et al.

FEMS microbiology reviews, 43(1), 88-107 (2018-11-13)

Many obligate or facultative intracellular bacteria pose a critical problem in clinical microbiology diagnosis as a result of their fastidious growth or lack of growth in conventional culture media. Molecular diagnosis is based on the analysis and demonstration of nucleic

Microbiota composition of the intestinal mucosa: association with fecal microbiota?

Arthur C Ouwehand et al.

Microbiology and immunology, 48(7), 497-500 (2004-07-24)

The fecal and mucosal microbiota of infants with rectal bleeding and the fecal microbiota of healthy age-matched controls were investigated by fluorescent in situ hybridization. Bifidobacteria were the main genus in both the feces and mucosa. The other genera tested

Pathogenic Escherichia coli.

James B Kaper et al.

Nature reviews. Microbiology, 2(2), 123-140 (2004-03-26)

Few microorganisms are as versatile as Escherichia coli. An important member of the normal intestinal microflora of humans and other mammals, E. coli has also been widely exploited as a cloning host in recombinant DNA technology. But E. coli is

Specific detection of Legionella pneumophila: construction of a new 16S rRNA-targeted oligonucleotide probe.

D Grimm et al.

Applied and environmental microbiology, 64(7), 2686-2690 (1998-07-02)

Based on comparative sequence analysis, we have designed an oligonucleotide probe complementary to a region of 16S rRNA of Legionella pneumophila which allows the differentiation of L. pneumophila from other Legionella species without cultivation. The specificity of the new probe

Spatial distribution of Escherichia coli in the mouse large intestine inferred from rRNA in situ hybridization.

L K Poulsen et al.

Infection and immunity, 62(11), 5191-5194 (1994-11-01)

Fluorescent oligonucleotide probes targeting rRNA were used to develop an in situ hybridization technique by which the spatial distribution of Escherichia coli in the large intestines of streptomycin-treated mice was determined. Single E. coli cells were identified in thin frozen

A commensal gone bad: complete genome sequence of the prototypical enterotoxigenic Escherichia coli strain H10407.

Lisa C Crossman et al.

Journal of bacteriology, 192(21), 5822-5831 (2010-08-31)

In most cases, Escherichia coli exists as a harmless commensal organism, but it may on occasion cause intestinal and/or extraintestinal disease. Enterotoxigenic E. coli (ETEC) is the predominant cause of E. coli-mediated diarrhea in the developing world and is responsible

Fluorescence in situ hybridisation for the identification and characterisation of prokaryotes.

Michael Wagner et al.

Current opinion in microbiology, 6(3), 302-309 (2003-07-02)

Fluorescence in situ hybridisation with rRNA-targeted nucleic acid probes can be used to directly identify microorganisms within complex samples in a few hours and therefore has widespread application in environmental and medical microbiology. The past year has seen significant methodological

Casein glycomacropeptide in the diet may reduce Escherichia coli attachment to the intestinal mucosa and increase the intestinal lactobacilli of early weaned piglets after an enterotoxigenic E. coli K88 challenge.

Rafael Gustavo Hermes et al.

The British journal of nutrition, 109(6), 1001-1012 (2012-08-02)

Casein glycomacropeptide (CGMP), a glycoprotein originating during cheese manufacture, has shown promising effects by promoting the growth of some beneficial bacteria in vitro, although its activity has not been well explored. The present study was designed to evaluate the effects

Growth and characterization of Escherichia coli DH5α biofilm on concrete surfaces as a protective layer against microbiologically influenced concrete deterioration (MICD).

Sahar Soleimani et al.

Applied microbiology and biotechnology, 97(3), 1093-1102 (2012-09-11)

Biofilms of selected bacteria strains were previously used on metal coupons as a protective layer against microbiologically influenced corrosion of metals. Unlike metal surfaces, concrete surfaces present a hostile environment for growing a protective biofilm. The main objective of this

Aberrant composition of gut microbiota of allergic infants: a target of bifidobacterial therapy at weaning?

P V Kirjavainen et al.

Gut, 51(1), 51-55 (2002-06-22)

Recent data have outlined a relationship between the composition of the intestinal microflora and allergic inflammation, and demonstrated the competence of probiotics in downregulation of such inflammation. Our aims were to characterise the relationship between gut microbes and the extent

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