Fluorescence in situ hybridisation for the identification and characterisation of prokaryotes.

Fluorescence in situ hybridisation for the identification and characterisation of prokaryotes.

Current opinion in microbiology (2003-07-02)

Michael Wagner, Matthias Horn, Holger Daims

PMID12831908

ABSTRACT

Fluorescence in situ hybridisation with rRNA-targeted nucleic acid probes can be used to directly identify microorganisms within complex samples in a few hours and therefore has widespread application in environmental and medical microbiology. The past year has seen significant methodological improvements in fluorescence in situ hybridisation, as well as in the combination of this method with other techniques for inferring functional traits of microorganisms within their environment.

MATERIALS

Product Number

Brand

Product Description

MBD0048

Sigma-Aldrich

Enterococcus faecium FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH), 20µM in water

MBD0047

Sigma-Aldrich

Enterococcus faecium FISH probe – ATTO 488, Probe for fluorescence in situ hybridization (FISH), 20µM in water

MBD0040

Sigma-Aldrich

Proteus FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH),20 μM in water

MBD0050

Sigma-Aldrich

Akkermansia muciniphila FISH probe-ATTO488, Probe for fluorescence in situ hybridization (FISH), 20µM in water

MBD0029

Sigma-Aldrich

Porphyromonas gingivalis FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH),20 μM in water

MBD0032

Sigma-Aldrich

Eubacteria FISH probe - ATTO488, Probe for fluorescence in situ hybridization (FISH), 20 µM in water

MBD0035

Sigma-Aldrich

Bacterial negative control FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH),20 μM in water

MBD0049

Sigma-Aldrich

Escherichia coli FISH probe -ATTO488, Probe for fluorescence in situ hybridization (FISH), 20µM in water

MBD0041

Sigma-Aldrich

Akkermansia muciniphila FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH),20 μM in water

MBD0059

Sigma-Aldrich

Alphaproteobacteria FISH probe-Cy3, Probe for fluorescence in situ hybridization (FISH), 20 μM in water

MBD0033

Sigma-Aldrich

Eubacteria FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH)

MBD0028

Sigma-Aldrich

Porphyromonas gingivalis FISH probe - ATTO488, Probe for fluorescence in situ hybridization (FISH)

MBD0034

Sigma-Aldrich

Bacterial negative control FISH probe - ATTO488, Probe for fluorescence in situ hybridization (FISH),20 μM in water

MBD0039

Sigma-Aldrich

Proteus FISH probe - ATTO488, Probe for fluorescence in situ hybridization (FISH),20 μM in water

MBD0042

Sigma-Aldrich

Escherichia coli FISH probe - Cy3, Probe for fluorescence in situ hybridization (FISH)