Merck
CN
Advanced Search
Chemical Structure Search
Search Within

pcr cyclers

Applied Filters:
Keyword:'pcr cyclers'
Showing 1-30 of 59 results for "pcr cyclers" within Papers
Arunkumar Arumugam et al.
Diagnostics (Basel, Switzerland), 10(10) (2020-09-30)
Quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay is the gold standard recommended to test for acute SARS-CoV-2 infection. However, it generally requires expensive equipment such as RNA isolation instruments and real-time PCR thermal cyclers. As a pandemic, COVID-19 has
Grace Wong et al.
PloS one, 10(7), e0131701-e0131701 (2015-07-07)
Many modern molecular diagnostic assays targeting nucleic acids are typically confined to developed countries or to the national reference laboratories of developing-world countries. The ability to make technologies for the rapid diagnosis of infectious diseases broadly available in a portable
Elena Sticchi et al.
Biochemical genetics, 42(3-4), 121-127 (2004-06-01)
In endothelial cells nitric oxide (NO) is synthesized by endothelial-nitric oxide synthase (e-NOS), constitutively expressed and encoded by a 26-exon gene, located on chromosome 7q35-36. The prevalence of the T rare variant of the G894T polymorphism in exon 7 of
Layla Mammo et al.
Clinical biochemistry, 39(7), 767-769 (2006-03-28)
To test reproducibility, speed and cost of testing for factor V Leiden and FII 20210G>A in our practice. We compared conformity, reproducibility, speed and cost using the Light Cycler (LC) and PCR-RFLP. There was 100% conformity and reproducibility. LC was
Elisa Bergamo et al.
Journal of virological methods, 181(1), 125-130 (2012-01-24)
The potential advantage of using fast PCR to detect human herpesvirus 8 (HHV-8) was tested by running a rapid cycling protocol (5s-steps) in one standard and two fast ramping thermal cyclers to evaluate the performance of 8 different fast reagents.
Götz Münchow et al.
Expert review of molecular diagnostics, 5(4), 613-620 (2005-07-15)
A chip-based PCR device is presented that is capable of rapid temperature ramping and handling sample volumes in the microliter range. The PCR chip comprises a microchannel thermally connected to three temperature zones. Inside this microchannel, the PCR sample plug
Bin Wu et al.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA, 22(6), 490-492 (2002-09-26)
To evaluate the feasibility of detecting low copy stem cell factor (SCF) mRNA in leukemic cells by in situ reverse transcriptase-PCR (RT-PCR). In situ RT-PCR was carried out on PE480 DNA Thermal Cycler to determine the expression of SCF mRNA
J M Benson et al.
Thrombosis and haemostasis, 86(5), 1188-1192 (2002-01-31)
Thrombophilia is a multigenic disease in which the combination of genetic polymorphisms increases the risk of deep vein thrombosis (DVT). The rapid identification of these genetic combinations requires high-throughput analysis of single nucleotide polymorphisms (SNPs). The TaqMan fluorogenic 5'-->*3' nuclease
Burkhard Malorny et al.
International journal of food microbiology, 117(2), 211-218 (2007-05-22)
A collaborative study including 13 German laboratories was conducted to evaluate the performance of two non-patented real-time PCR methods for the detection of Salmonella in milk powder targeting the ttrC/ttrA- or the invA gene. The enrichment procedure and sample DNA
Bożena Nejman-Faleńczyk et al.
Toxins, 7(11), 4745-4757 (2015-11-19)
A novel procedure for the detection of specific DNA sequences has been developed. This procedure is based on the already known method employing PCR with appropriate primers and a sequence-specific DNA probe labeled with the fluorescent agent 6-carboxylfluorescein (FAM) at
Senne Cornelis et al.
Scientific reports, 9(1), 7341-7341 (2019-05-16)
The demand to perform forensic DNA profiling outside of centralized laboratories and on the crime scene is increasing. Several criminal investigations would benefit tremendously from having DNA based information available in the first hours rather than days or weeks. However
Arpasiri Srisrattakarn et al.
World journal of microbiology & biotechnology, 36(11), 162-162 (2020-09-30)
Methicillin-resistant staphylococci (MRS) are important antimicrobial-resistant pathogens in sepsis. Conventional blood cultures take 24-72 h. The polymerase chain reaction (PCR)-based methods give faster results (2-3 h) but need expensive thermal cyclers. We therefore developed an isothermal recombinase polymerase amplification (RPA) combined with
Weam I Zaky et al.
PLoS neglected tropical diseases, 12(11), e0006962-e0006962 (2018-11-22)
Currently, molecular xenomonitoring efforts for lymphatic filariasis rely on PCR or real-time PCR-based detection of Brugia malayi, Brugia timori and Wuchereria bancrofti in mosquito vectors. Most commonly, extraction of DNA from mosquitoes is performed using silica column-based technologies. However, such
Fatma Y Beyaztas et al.
Saudi medical journal, 28(10), 1493-1495 (2007-10-05)
To examine the allele frequencies of HumFABP2 locus in 155 individuals from different regions of Turkey. The study was carried out in Cumhuriyet University Hospital, Sivas, Turkey, between March and June 2006. The allele and genotype frequencies for HumFABP2 were
Ahmet Irdem et al.
Saudi medical journal, 26(4), 580-583 (2005-05-19)
To determine the prevalence of factor V Leiden (FVL) and prothrombin gene (PG) 20210A mutations in patients who attended the outpatient clinic and do not have a family history of thrombosis. We researched FVL and PG20210A mutations in 151 outpatients
S Sahin et al.
Genetics and molecular research : GMR, 10(4), 3987-3991 (2011-12-20)
We analyzed distribution of HLA-B27 and CYP2D6*4 mutations in 249 patients from Tokat province in Turkey with symptoms of arthritis, sacroiliac, joint and back pain, using a LightCycler 480 II Real-Time PCR thermal cycler. The Genes-4U was applied for studying
Bakhouche Houcher et al.
Pediatric neurosurgery, 45(6), 472-477 (2010-02-18)
Neural tube defects (NTD) are severe congenital malformations due to a failure in neural tube formation at the beginning of pregnancy. The etiology of NTD is multifactorial, with environmental and genetic determinants. We suggest a study of gene-gene interactions regarding
Robert S McLaren et al.
Forensic science international. Genetics, 13, 195-205 (2014-09-03)
The PowerPlex(®) ESI 16 Fast, ESI 17 Fast, ESX 16 Fast, and ESX 17 Fast Systems represent faster cycling versions (50min or less) of the PowerPlex(®) ESI and ESX Systems released by Promega in 2009 to accommodate the ENFSI and
D W Kim
Experimental & molecular medicine, 33(1 Suppl), 101-109 (2001-11-16)
So far, quantitative techniques, such as PCR and FISH, have been used to detect of DNA and RNA. However, it is difficult to measure and compare the exact amount of amplified products with the results of endpoint analysis in conventional
S Syrjänen et al.
Sexually transmitted diseases, 19(3), 140-145 (1992-05-01)
Polymerase chain reaction (PCR) was used to produce biotinylated DNA probes for human papillomavirus (HPV) types 16 and 18. The specificity and sensitivity of the probes were tested with in situ hybridization to detect HPV DNA in cervical biopsies or
Abdullah T Demiryurek et al.
Current eye research, 35(12), 1128-1134 (2010-10-22)
To analyze the genotype distributions and allele frequencies for ROCK2 Thr431Asn and Arg83Lys polymorphisms among the diabetic retinopathy patients in a Turkish population. In this case-control study, 335 patients with diabetes mellitus were recruited and divided into three groups according
Johannes Wöhrle et al.
Scientific reports, 10(1), 5770-5770 (2020-04-03)
In this work we show how DNA microarrays can be produced batch wise on standard microscope slides in a fast, easy, reliable and cost-efficient way. Contrary to classical microarray generation, the microarrays are generated via digital solid phase PCR. We
M C Z Novaretti et al.
Immunohematology, 26(2), 66-70 (2010-10-12)
The paucity of appropriate reagents for serologic typing of the Diego blood group antigens has prompted the development of a real-time PCR and melting curve analysis for Diego blood group genotyping. In this study, we phenotyped 4326 donor blood samples
Isabel Ferreirós-Vidal et al.
The Journal of rheumatology, 30(1), 102-104 (2003-01-01)
To investigate whether the 3 most common mutations in the NOD2 gene that confer susceptibility to Crohn's disease (CD) are also associated with ankylosing spondylitis (AS). DNA from 112 patients with AS and 168 controls of homogenous Spanish ancestry were
F S Gittinger et al.
Tissue antigens, 60(1), 64-70 (2002-10-09)
The granulocyte antigens HNA-1a, -1b and -1c reside on the FcgammaIIIb receptor, which is exclusively expressed on neutrophils. They are involved in autoimmune and alloimmune neutropenias as well as in severe transfusion reactions. Recent family studies show the HNA-1c antigen
Yangyang Jiang et al.
The Analyst, 143(16), 3798-3807 (2018-06-29)
Unlike conventional approaches that require bulky and expensive pumping equipment, herein, we present a simple method for self-activated microdroplet generation and transport inside a long microchannel. The high gas-pressure in the syringes is used to provide the built-in power of
Gregory Day et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 27(6), 807-813 (2008-11-20)
Reduced SHOX gene expression has been demonstrated to be associated with all skeletal abnormalities in Turner syndrome, other than scoliosis (and kyphosis). There is evidence to suggest that Turner syndrome scoliosis is clinically and radiologically similar to idiopathic scoliosis, although
Grittaya Srimongkol et al.
Scientific reports, 10(1), 7768-7768 (2020-05-10)
Staphylococcus aureus strains carrying enterotoxin A gene (sea) causes food poisoning and cannot be distinguished from non-pathogenic strains by the culture method. Here, we developed a rapid, specific and sensitive visual detection of sea using loop-mediated isothermal amplification (LAMP) combined
Kejia Cao et al.
Investigative ophthalmology & visual science, 61(3), 13-13 (2020-03-17)
To investigate the efficacy of intravitreal administration of resveratrol (RSV) in a microbead-induced high intraocular pressure (IOP) murine model for glaucoma. Experiments were performed using adult C57BL/6JJcl mice. Polystyrene microbeads were injected into the anterior chamber to induce IOP elevation.
Mehmet E Kalender et al.
Oncology research, 18(11-12), 583-591 (2010-10-14)
The objective of this study was to analyze the genotype distributions and allele frequencies for ROCK2 Thr431Asn and Arg83Lys polymorphisms among breast cancer patients. In this case-control study, 223 patients with breast cancer were recruited and divided into two groups
Page 1 of 2