using a repeat pipette and 0.5 mL pipette tip. Cover sample wells with
adhesive film strips.
b. Transfer 1.0 mL of Wash Solution to additional sample wells (1 well/sample) using a repeat pipette and 10
Protease and Phosphatase inhibitors.
3. Shaker.
4. Precision pipettes to deliver 2 µL to 1 mL volumes.
5. Adjustable 1-25 mL pipettes for reagent
preparation.
6. 100 mL and 1 liter graduated cylinders
using a
repeat pipette and 10 mL pipette tips.
3. Transfer 45 µL of Resuspension Buffer Tq to the sample wells in the resuspension plate using a repeat
pipette and a 0.5 mL pipette tip. Cover
Protease and Phosphatase inhibitors.
3. Shaker.
4. Precision pipettes to deliver 2 µl to 1 ml volumes.
5. Adjustable 1-25 ml pipettes for reagent preparation.
6. 100 ml and 1 liter graduated cylinders
using a
repeat pipette and 10 mL pipette tips.
c. Transfer 45 µL of Resuspension Buffer Tq to the sample wells in the resuspension plate using a repeat
pipette and a 0.5 mL pipette tip. Cover
) using a
repeat pipette and 10 mL pipette tips.
c. Transfer 45 µL of Resuspension Buffer Tq to the sample wells in the resuspension plate using a repeat
pipette and a 0.5 mL pipette tip.
Protease and Phosphatase inhibitors.
3. Shaker.
4. Precision pipettes to deliver 2 µL to 1 mL volumes.
5. Adjustable 1-25 mL pipettes for reagent
preparation.
6. 100 mL and 1 liter graduated cylinders
Protease and Phosphatase inhibitors.
3. Shaker.
4. Precision pipettes to deliver 2 µL to 1 mL volumes.
5. Adjustable 1–25 mL pipettes for reagent
preparation.
6. 100 mL and 1 liter graduated cylinders
antibody with a pipette via the
injection/recovery port or flush to waste by applying
vacuum.
4. Fill the wash reservoir with wash solution, then flush
by applying vacuum. Repeat wash step two more
Protease and Phosphatase inhibitors
• Shaker
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 L graduated
Protease and Phosphatase inhibitors.
3. Shaker.
4. Precision pipettes to deliver 2 L to 1 mL volumes.
5. Adjustable 1-25 mL pipettes for reagent
preparation.
6. 100 mL and 1 liter graduated cylinders
Protease and Phosphatase inhibitors
3. Shaker
4. Precision pipettes to deliver 2 L to 1 mL volumes
5. Adjustable 1-25 mL pipettes for reagent preparation
6. 100 mL and 1 liter graduated cylinders
using a repeat pipette and a 0.5 mL
pipette tip, into the GDS sample wells (1 well/sample). Cover sample wells with adhesive film strips.
2. Add 1 mL, using a repeat pipette and a 10 mL pipette tip
using a repeat pipette and a 0.5 mL pipette tip. Cover sample wells
with adhesive strips.
2. Transfer 1.0 mL of Wash Solution to additional sample wells (1 well/sample) using a repeat pipette and
a
sample) using repeat pipette and 10 mL pipette tips.
c. Transfer 45 µL of Resuspension Buffer Tq to the sample wells in the resuspension plate using a repeat
pipette and a 0.5 mL pipette tip. Cover
well/ sample) using a repeat pipette and 0.5 mL pipette tip. Add 0.7 mL
of Wash Solution to sample wells containing Concentration Reagent using a repeat pipette and 10 mL
pipette tip. Cover sample wells
Protease and Phosphatase inhibitors.
3. Shaker.
4. Precision pipettes to deliver 2 µL to 1 mL volumes.
5. Adjustable 1-25 mL pipettes for reagent
preparation.
6. 100 mL and 1 liter graduated cylinders
using a repeat pipette and 0.5 mL pipette tips. Cover sample
wells with adhesive strips.
b. Transfer 1 mL of Wash Solution to an additional sample wells (1 well/sample) using a repeat pipette and
1000 micropipette. Break organoids by placing the
pipette tip perpendicular to the bottom of the well and expel organoids with a scraping motion.
Repeat 10 times.
6. Transfer the organoid suspension
media.
2. Using a P1000 pipette (Note: Do not use a serological pipette), resuspend the cell pellet in 600 μL expansion
medium. Pipette up and down 50 times with the pipette tip touching the bottom of
Protease and Phosphatase inhibitors.
3. Shaker.
4. Precision pipettes to deliver 2 µL to 1 mL volumes.
5. Adjustable 1-25 mL pipettes for reagent
preparation.
6. 100 mL and 1 liter graduated cylinders