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  • Cell-wall associated polysaccharide from the psychrotolerant bacterium Psychrobacter arcticus 273-4: isolation, purification and structural elucidation.

Cell-wall associated polysaccharide from the psychrotolerant bacterium Psychrobacter arcticus 273-4: isolation, purification and structural elucidation.

Extremophiles : life under extreme conditions (2019-07-17)
Angela Casillo, Annarita Ricciardelli, Ermenegilda Parrilli, Maria Luisa Tutino, Maria Michela Corsaro
ABSTRACT

In this paper, the structure of the capsular polysaccharide isolated from the psychrotolerant bacterium Psychrobacter arcticus 273-4 is reported. The polymer was purified by gel filtration chromatography and the structure was elucidated by means of one- and two-dimensional NMR spectroscopy, in combination with chemical analyses. The polysaccharide consists of a trisaccharidic repeating unit containing two residues of glucose and a residue of a N,N-diacetyl-pseudaminic acid.

MATERIALS
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Sigma-Aldrich
Sephacryl®, 400-HR, MW range 20-8000 kDa (globular proteins)