- An electron transfer path connects subunits of a mycobacterial respiratory supercomplex.
An electron transfer path connects subunits of a mycobacterial respiratory supercomplex.
Science (New York, N.Y.) (2018-10-27)
Hongri Gong, Jun Li, Ao Xu, Yanting Tang, Wenxin Ji, Ruogu Gao, Shuhui Wang, Lu Yu, Changlin Tian, Jingwen Li, Hsin-Yung Yen, Sin Man Lam, Guanghou Shui, Xiuna Yang, Yuna Sun, Xuemei Li, Minze Jia, Cheng Yang, Biao Jiang, Zhiyong Lou, Carol V Robinson, Luet-Lok Wong, Luke W Guddat, Fei Sun, Quan Wang, Zihe Rao
PMID30361386
ABSTRACT
We report a 3.5-angstrom-resolution cryo-electron microscopy structure of a respiratory supercomplex isolated from Mycobacterium smegmatis. It comprises a complex III dimer flanked on either side by individual complex IV subunits. Complex III and IV associate so that electrons can be transferred from quinol in complex III to the oxygen reduction center in complex IV by way of a bridging cytochrome subunit. We observed a superoxide dismutase-like subunit at the periplasmic face, which may be responsible for detoxification of superoxide formed by complex III. The structure reveals features of an established drug target and provides a foundation for the development of treatments for human tuberculosis.
MATERIALS
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BrdU (5-Bromo-2′-deoxyuridine), Used for the study of DNA synthesis where it is incorporated into DNA in place of thymidine, BrdU can be used in conjunction with anti-BrdU for immunocytochemical analysis of cell proliferation.