Transcriptomic Analysis in the Sea Anemone Nematostella vectensis.

Transcriptomic Analysis in the Sea Anemone Nematostella vectensis.

Methods in molecular biology (Clifton, N.J.) (2020-10-20)

Jacob F Warner, Eric Röttinger

ABSTRACT

The sea anemone Nematostella vectensis is an emerging research model to study embryonic development and regeneration at the molecular and global transcriptomic level. Transcriptomics analysis is now routinely used to detect differential expression at the genome level. Here we present the latest procedures for isolating high-quality RNA required for next generation sequencing, as well as methods and resources for quantifying transcriptomic data.

MATERIALS

Product Number

Brand

Product Description

C2432

Sigma-Aldrich

Chloroform, contains 100-200 ppm amylenes as stabilizer, ≥99.5%

P2069

Sigma-Aldrich

Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with 10 mM Tris, pH 8.0, 1 mM EDTA, Supplied with Equilibration buffer, for molecular biology