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Biotinylated Micro-RNA Pull Down Assay for Identifying miRNA Targets.

Bio-protocol (2017-05-05)
Pornima Phatak, James M Donahue
ABSTRACT

microRNA (miRNA) directly associates with its target transcripts (mRNA). This protocol describes a method for detection of direct interaction between miRNA and mRNA. The result of interaction helps screening the specific target mRNAs for a miRNA.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
IGEPAL® CA-630, Molecular Biology
Sigma-Aldrich
Chloroform, contains 100-200 ppm amylenes as stabilizer, ≥99.5%
Sigma-Aldrich
2-Propanol, BioReagent, ≥99.5%, Molecular Biology
Sigma-Aldrich
Ethyl alcohol, Pure 200 proof, Molecular Biology