Oncogenic Transformation of Human-Derived Gastric Organoids.

Oncogenic Transformation of Human-Derived Gastric Organoids.

Methods in molecular biology (Clifton, N.J.) (2016-08-20)

Nina Bertaux-Skeirik, Jomaris Centeno, Jian Gao, Joel Gabre, Yana Zavros

ABSTRACT

The culture of organoids has represented a significant advancement in the gastrointestinal research field. Previous research studies have described the oncogenic transformation of human intestinal and mouse gastric organoids. Here we detail the protocol for the oncogenic transformation and orthotopic transplantation of human-derived gastric organoids.

MATERIALS

Product Number

Brand

Product Description

C9891

Sigma-Aldrich

Collagenase from Clostridium histolyticum, Type IA, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid, For general use

Y0503

Sigma-Aldrich

Y-27632 dihydrochloride, ≥98% (HPLC)

H3884

Sigma-Aldrich

Hyaluronidase from bovine testes, Type IV-S, lyophilized powder (essentially salt-free), 750-3000 units/mg solid

H0887

Sigma-Aldrich

HEPES solution, 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

A9165

Sigma-Aldrich

N-Acetyl-L-cysteine, BioReagent, suitable for cell culture