- Generation of human iPS cell line CTL07-II from human fibroblasts, under defined and xeno-free conditions.
Generation of human iPS cell line CTL07-II from human fibroblasts, under defined and xeno-free conditions.
Stem cell research (2016-10-30)
Malin Kele, Kelly Day, Harriet Rönnholm, Jens Schuster, Niklas Dahl, Anna Falk
PMID27789397
ABSTRACT
CTL07-II is a healthy feeder-free and characterized human induced pluripotent stem (iPS) cell line. Cultured under xeno-free and defined conditions. The line is generated from healthy human fibroblasts with non-integrating Sendai virus vectors encoding the four Yamanaka factors, OCT4, SOX2, KLF4 and cMYC. The generated iPS cells are free from reprogramming vectors and their purity, karyotypic stability and pluripotent capacity is confirmed.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
ROCK Inhibitor (Y-27632), The ROCK Inhibitor (Y-27632) is available in a 5 mg format & has been optimized & validated for cell culture & Neuroscience applications.