- Detection of Tsh protein mucinolytic activity by SDS-PAGE.
Detection of Tsh protein mucinolytic activity by SDS-PAGE.
Journal of microbiological methods (2006-12-13)
Renata K T Kobayashi, Luis Carlos J Gaziri, Emerson J Venancio, Marilda C Vidotto
PMID17157399
ABSTRACT
The temperature-sensitive hemagglutinin (Tsh, 140 kDa) produced by Escherichia coli is cleaved into a fragment (106 kDa) containing mucinase activity, and an agglutinin fragment (33 kDa). By incorporating mucins into SDS-PAGE gels stained by Schiff's periodic acid, we could simultaneously detect about 0.5 microg of mucinase activity and the fragment molecular mass.
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