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  • An enzyme-responsive nanocontainer as an intelligent signal-amplification platform for a multiple proteases assay.

An enzyme-responsive nanocontainer as an intelligent signal-amplification platform for a multiple proteases assay.

Chemical communications (Cambridge, England) (2012-10-10)
Xinjian Yang, Fang Pu, Cuie Chen, Jinsong Ren, Xiaogang Qu
ABSTRACT

Based on selective pore-opening in the presence of protease, we have developed a novel signal amplification assay for multiple proteases detection and their inhibition using protein-capped mesoporous scaffolding as the substrate.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Glucose Oxidase from Aspergillus niger, Type X-S, lyophilized powder, 100,000-250,000 units/g solid (without added oxygen)
Sigma-Aldrich
Glucose Oxidase from Aspergillus niger, Type VII, lyophilized powder, ≥100,000 units/g solid (without added oxygen)
Sigma-Aldrich
Phenylmethanesulfonyl fluoride, ≥99.0% (T)
Sigma-Aldrich
Phenylmethanesulfonyl fluoride, ≥98.5% (GC)
Sigma-Aldrich
Phenylmethanesulfonyl fluoride solution, ~0.1 M in ethanol (T)