- White mutants of chloroperoxidase-secreting Caldariomyces fumago as superior production strains, revealing an interaction between pigmentation and enzyme secretion.
White mutants of chloroperoxidase-secreting Caldariomyces fumago as superior production strains, revealing an interaction between pigmentation and enzyme secretion.
Applied and environmental microbiology (2012-05-29)
Markus Buchhaupt, Sonja Hüttmann, Jens Schrader
PMID22636011
ABSTRACT
By mutant-colony screening of Caldariomyces fumago, several white mutants were isolated that are superior strains for the production of the valuable enzyme chloroperoxidase (CPO). Their culture supernatant lacks the contaminating dark pigment, which simplifies downstream processing. Furthermore, the CPO content increased significantly faster than the wild-type rate, which uncovers possible interactions between pigmentation and enzyme secretion.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Chloroperoxidase from Caldariomyces fumago, buffered aqueous suspension, ≥3,000 units/mL
Sigma-Aldrich
Chloroperoxidase from Caldariomyces fumago, buffered aqueous suspension, 1,000-2,000 units/mg protein (E1%/280)
Sigma-Aldrich
Chloroperoxidase from Caldariomyces fumago, aqueous suspension, brown, >10,000 U/mL