- Improvement of the plaque technique for human rotaviruses: effect of fetal bovine serum, acetyltrypsin and agar.
Improvement of the plaque technique for human rotaviruses: effect of fetal bovine serum, acetyltrypsin and agar.
The Kurume medical journal (1991-01-01)
Y Imamura, S Yamamoto, M Shingu
PMID1668675
ABSTRACT
Dilution of viruses, which were previously treated with 10 micrograms acetyltrypsin/ml, in MEM supplemented with 2.5% fetal bovine serum caused little damage of cell monolayers resulting in the formation of discrete plaques. When agar and acetyltrypsin were added to the overlay medium at the concentrations of 0.8% and 5 micrograms/ml, respectively, clear plaques were induced. Linear relationships were obtained between virus concentration and the number of plaques, thus the procedure described in this report might be used for reliable plaque assay of rotaviruses. Moreover, these conditions for plaque formation were successful for estimating isolated viruses, as well as standard strains.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Trypsin from human pancreas, salt-free, lyophilized powder, vial of ≥1,000 BAEE units
Sigma-Aldrich
Trypsin from bovine pancreas, TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein
Sigma-Aldrich
Trypsin from bovine pancreas, TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein
Sigma-Aldrich
Trypsin from bovine pancreas, essentially salt-free, lyophilized powder, ≥9,000 BAEE units/mg protein, BioReagent, suitable for cell culture
Sigma-Aldrich
Trypsin from bovine pancreas, ≥2,500 USP units/mg solid, meets USP testing specifications
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Trypsin from bovine pancreas, Type XI, lyophilized powder, ≥6,000 BAEE units/mg protein
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TrypZean® bovine, recombinant, expressed in corn, lyophilized powder, ≥3,350 units/mg solid (USP)
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Trypsin solution from porcine pancreas, sterile-filtered, BioReagent, suitable for cell culture, 25 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red
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Trypsin from porcine pancreas, lyophilized powder, Type II-S, 1,000-2,000 units/mg dry solid
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Trypsin from porcine pancreas, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture, 1,000-2,000 BAEE units/mg solid
Sigma-Aldrich
Trypsin solution from porcine pancreas, 1 ×, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red
Sigma-Aldrich
Trypsin solution from porcine pancreas, sterile-filtered, BioReagent, suitable for cell culture, 25 g porcine trypsin per liter in 0.9% sodium chloride
Sigma-Aldrich
Trypsin from porcine pancreas, Type IX-S, lyophilized powder, 13,000-20,000 BAEE units/mg protein
Sigma-Aldrich
Trypsin from porcine pancreas, lyophilized powder, BioReagent, suitable for cell culture, 1,000-2,000 BAEE units/mg solid