[Determination of malachite green and leukomalachite green residues in fish muscle by high-performance liquid chromatography method].

Malachite green (MG) and leukomalachite green (LMG) are subjected to monitoring fish muscle, with a minimum required performance limit (MRPL) set 2 microg/kg. Samples were extracted with acetonitryle-buffer mixture and cleaned up on SCX solid phase extraction (SPE) column. LC separation MG and LMG was done on column Luna Phenyl-Hexyl Phenomenex. Samples were fortified with MG and LMG between 2 - 25 microg/kg. The coefficients of variation (CV%) were lower than 14% for MG and 16%for LMG. The mean recoveries were in the range 65- 83% for MG and 70- 73% for LMG. This method fulfils the criteria for identification and determination of MG andLMG residues in the samples of fish muscle.