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DNA extraction from keratin and chitin.

Methods in molecular biology (Clifton, N.J.) (2012-01-13)
Paula F Campos, Thomas M P Gilbert
ABSTRACT

DNA extracted from keratinous and chitinous materials can be a useful source of genetic information. To effectively liberate the DNA from these materials, buffers containing relatively high levels of DTT, proteinase K, and detergent are recommended, followed by purification using either silica-column or organic methods.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
1,4-Dithioerythritol, ≥99.0%
Sigma-Aldrich
1,4-Dithioerythritol, BioReagent, Molecular Biology, ≥99.0%
Sigma-Aldrich
1,4-Dithioerythritol, BioXtra, ≥99.0%
Sigma-Aldrich
1,4-Dithioerythritol, ≥99.0% (RT), BioUltra