- Layer by layer assembly of biotinylated protein networks for signal amplification.
Layer by layer assembly of biotinylated protein networks for signal amplification.
Chemical communications (Cambridge, England) (2013-01-19)
Yu W Chu, Bo Y Wang, Huei-Shian Lin, Tai-Yen Lin, Yuan-Jen Hung, David A Engebretson, Wonjae Lee, James R Carey
PMID23329177
ABSTRACT
Described herein is a unique and inexpensive method that outperforms commercial methods that amplify the streptavidin-biotin recognition event. Amplification induced by streptavidin and biotinylated protein causes the formation of a large detectable polymer. This approach enjoys a 100-fold decrease in detection limit in comparison with the commercial methods.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Streptavidin from Streptomyces avidinii, affinity purified, lyophilized from 10 mM potassium phosphate, ≥13 U/mg protein
Sigma-Aldrich
Avidin from egg white, recombinant, expressed in corn, ≥12 units/mg protein (E1%/280)
Sigma-Aldrich
Streptavidin from Streptomyces avidinii, recombinant, expressed in E. coli, lyophilized powder
Sigma-Aldrich
Streptavidin from Streptomyces avidinii, essentially salt-free, lyophilized powder, ≥13 units/mg protein