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  • Surface modification of biomaterials for conjugation with human fetal osteoblasts.

Surface modification of biomaterials for conjugation with human fetal osteoblasts.

Chimia (2013-08-24)
Françoise Borcard, Phally Kong, Céline Journot, Davide Staedler, Philip N Sturzenegger, Franziska Krauss Juillerat, Urs T Gonzenbach, Lucienne Juillerat-Jeanneret, Sandrine Gerber-Lemaire
ABSTRACT

Surface functionalization of hydroxyapatite (HA) and beta-tricalcium phosphate (TCP) bioceramics with chemical ligands containing a pyrrogallol moiety was developed to improve the adhesion of bone cell precursors to the biomaterials. Fast and biocompatible copper-free click reaction with azido-modified human fetal osteoblasts resulted in improved cell binding to both HA and TCP bioceramics, opening the way for using this methodology in the preparation of cell-engineered bone implants.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Hydroxyapatite, purum p.a., ≥90% (as Ca3(PO4)2, KT)
Sigma-Aldrich
Hydroxyapatite, puriss., meets analytical specification of Ph. Eur., BP, FCC, E341, ≥90% (calculated on glowed substance)