A novel modified carbon paste electrode based on NiO/CNTs nanocomposite and (9, 10-dihydro-9, 10-ethanoanthracene-11, 12-dicarboximido)-4-ethylbenzene-1, 2-diol as a mediator for simultaneous determination of cysteamine, nicotinamide adenine dinucleotide and folic acid.

A novel modified carbon paste electrode based on NiO/CNTs nanocomposite and (9, 10-dihydro-9, 10-ethanoanthracene-11, 12-dicarboximido)-4-ethylbenzene-1, 2-diol as a mediator for simultaneous determination of cysteamine, nicotinamide adenine dinucleotide and folic acid.

Biosensors & bioelectronics (2013-05-28)

Hassan Karimi-Maleh, Pourya Biparva, Mehdi Hatami

ABSTRACT

A carbon paste electrode (CPE) modified with (9, 10-dihydro-9, 10-ethanoanthracene-11, 12-dicarboximido)-4-ethylbenzene-1, 2-diol (DEDE) and NiO/CNTs nanocomposite was used for the sensitive voltammetric determination of cysteamine (CA), nicotinamide adenine dinucleotide (NADH) and folic acid (FA) for the first time. The synthesized materials were characterized with different methods such as XRD, cyclic voltammetry, electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV). The modified electrode exhibited a potent and persistent electron mediating behavior followed by well-separated oxidation peaks of CA, NADH and FA. The peak currents were linearly dependent on CA, NADH and FA concentrations using square wave voltammetry (SWV) method in the ranges of 0.01-250, 1.0-500, and 3.0-550 µmol L⁻¹, with detection limits of 0.007, 0.6, and 0.9 µmol L⁻¹, respectively. The modified electrode was used for the determination of CA, NADH and FA in biological and pharmaceutical samples.

MATERIALS

Product Number

Brand

Product Description

399523

Sigma-Aldrich

Nickel(II) oxide, green, −325 mesh, 99%

710725

Sigma-Aldrich

Ethylbenzene solution, suitable for NMR (reference standard), 5% in chloroform-d (99.8 atom % D), TMS 1 %, NMR tube size 5 mm × 7 in.

637130

Sigma-Aldrich

Nickel(II) oxide, nanopowder, <50 nm particle size (TEM), 99.8% trace metals basis

30070

Sigma-Aldrich

Cysteamine, ≥98.0% (RT)

487120

Sigma-Aldrich

Ethylbenzene solution, suitable for NMR (reference standard), 0.1% in chloroform-d (99.8 atom % D), TMS 0.01 %, NMR tube size 8 mm × 8 in.

M9768

Sigma-Aldrich

Cysteamine, ~95%

714917

Sigma-Aldrich

Ethylbenzene solution, suitable for NMR (reference standard), 0.1% in chloroform-d (99.8 atom % D), TMS 0.01 %, NMR tube size 5 mm × 8 in. , ultra-thin wall

708046

Sigma-Aldrich

Ethylbenzene solution, suitable for NMR (reference standard), 10% in chloroform-d (99.8 atom % D)

708054

Sigma-Aldrich

Ethylbenzene solution, suitable for NMR (reference standard), 0.1% in chloroform-d ("100%", 99.96 atom % D)

203882

Sigma-Aldrich

Nickel(II) oxide, 99.99% trace metals basis

551341

Sigma-Aldrich

Ethylbenzene solution, suitable for NMR (reference standard), 5% in chloroform-d (99.8 atom % D), TMS 2 %, NMR tube size 5 mm × 8 in.

717959

Sigma-Aldrich

Ethylbenzene solution, suitable for NMR (reference standard), 1% in chloroform-d (99.8 atom % D)

733768

Sigma-Aldrich

Ethylbenzene solution, suitable for NMR (reference standard), 10% in chloroform-d (99.8 atom % D), NMR tube size 10 mm × 8 in.

717967

Sigma-Aldrich

Ethylbenzene solution, suitable for NMR (reference standard), 0.1% in chloroform-d (99.8 atom % D), TMS 0.1 %

612480

Supelco

Ethylbenzene solution, suitable for NMR (reference standard), 0.1% in chloroform-d (99.8 atom % D), chromium(III) acetylacetonate 0.1 mg/mL, TMS 0.01 %, NMR tube size 5 mm × 8 in.

487104

Supelco

Ethylbenzene solution, suitable for NMR (reference standard), 0.1% in chloroform-d (99.8 atom % D), TMS 0.01 %, NMR tube size 5 mm × 8 in.

736198

Supelco

Ethylbenzene solution, suitable for NMR (reference standard), 10% in chloroform-d (99.8 atom % D), NMR tube size 5 mm × 8 in. , ultra-thin wall

M6500

Sigma-Aldrich

Cysteamine hydrochloride, ≥98% (titration)

30078

Sigma-Aldrich

Cysteamine hydrochloride, BioXtra