- Studies on extraction of mannanase enzyme by Aspergillus terreus SUK-1 from fermented palm kernel cake.
Studies on extraction of mannanase enzyme by Aspergillus terreus SUK-1 from fermented palm kernel cake.
Pakistan journal of biological sciences : PJBS (2014-02-08)
Jahwarhar Izuan Abd Rashid, Noraini Samat, Wan Mohtar Wan Yusoff
PMID24502150
ABSTRACT
Microbial mannanases have become biotechnologically important in industry but their application is limited due to high production cost. In presents study, the extraction of mannanase from fermented Palm Kernel Cake (PKC) in the Solid State Fermentation (SSF) was optimized. Local isolate of Aspergillus terreus SUK-1 was grown on PKC in (SSF) using column bioreactor. The optimum condition were achieved after two washes of fermented PKC by adding of 10% glycerol (v/v) soaked for 10 h at the room temperature with solvent to ratio, 1:5 (w/v).
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Glycerol solution, puriss., meets analytical specification of Ph. Eur., BP, 84-88%
Sigma-Aldrich
Glycerol, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for electrophoresis, ≥99% (GC)