- Combinatorial alanine-scanning.
Combinatorial alanine-scanning.
Current opinion in chemical biology (2001-08-02)
K L Morrison, G A Weiss
PMID11479122
ABSTRACT
Combinatorial libraries of alanine-substituted proteins can be used to rapidly identify residues important for protein function, stability and shape. Each alanine substitution examines the contribution of an individual amino acid sidechain to the functionality of the protein. The recently described method of shotgun scanning uses phage-displayed libraries of alanine-substituted proteins for high-throughput analysis.
MATERIALS
Product Number
Brand
Product Description
Supelco
L-Alanine, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
L-Alanine, from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Alanine, European Pharmacopoeia (EP) Reference Standard