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  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2-Mercaptoethanol, BioUltra, Molecular Biology, ≥99.0% (GC)
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Formaldehyde solution, ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)
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Piperidine, ReagentPlus®, 99%
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Acetone, ACS reagent, ≥99.5%
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Sodium chloride, Vetec, reagent grade, 99%
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Acetone, Laboratory Reagent, ≥99.5%
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Tetrahydrofuran, contains 200-400 ppm BHT as inhibitor, ACS reagent, ≥99.0%
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Piperidine, biotech. grade, ≥99.5%
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1-Octanol, ACS reagent, ≥99%
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1-Octanol, ReagentPlus®, 99%
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N,N-Dimethylformamide, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.8% (GC)
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Acetone, ACS reagent, ≥99.5%
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Acetone, puriss., meets analytical specification of Ph. Eur., BP, NF, ≥99% (GC)
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Acetone, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)
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Methanol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)
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Diethylene glycol, Pharmaceutical Secondary Standard; Certified Reference Material
USP
Acetone, United States Pharmacopeia (USP) Reference Standard
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Acetone, natural, ≥97%
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Formaldehyde-12C solution, 20% in H2O, 99.9 atom % 12C
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Sodium chloride-35Cl, 99 atom % 35Cl
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Sodium chloride, random crystals, 99.9% trace metals basis
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Methanol, suitable for NMR (reference standard)
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2-Mercaptoethanol, ≥99.0%
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Tetrahydrofuran, anhydrous, ≥99.9%, inhibitor-free
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1-Octanol, anhydrous, ≥99%
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1-Octanol, ≥98%, FCC, FG
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Acetone, analytical standard
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1-Octanol, analytical standard
Sigma-Aldrich
Diethylene glycol, BioUltra, ≥99.0% (GC)