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  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Supelco
2-Mercaptoethanol, derivatization grade (HPLC), LiChropur, ≥99.0% (GC)
SAFC
Formaldehyde solution, contains 10-15% methanol as stabilizer, 37 wt. % in H2O
Sigma-Aldrich
Acetone, Laboratory Reagent, ≥99.5%
Sigma-Aldrich
Tetrahydrofuran, ≥99.0%, contains 200-400 ppm BHT as inhibitor, ReagentPlus®
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N,N-Dimethylformamide, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.8% (GC)
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Acetone, puriss., meets analytical specification of Ph. Eur., BP, NF, ≥99% (GC)
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Methanol, ACS reagent, ≥99.8%
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Diethyl ether, puriss., contains ~5 mg/L 2,6-di-tert.-butyl-4-methylphenol as stabilizer, meets analytical specification of Ph. Eur., BP, ≥99.5% (GC)
Sigma-Aldrich
Chloroform, contains amylenes as stabilizer, ACS reagent, ≥99.8%
USP
Acetone, United States Pharmacopeia (USP) Reference Standard
Supelco
Piperidine, analytical standard
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Sodium chloride, Vetec, reagent grade, 99%
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Acetone, suitable for HPLC, ≥99.8%
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Methanol, suitable for HPLC, gradient grade, suitable as ACS-grade LC reagent, ≥99.9%
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Sodium chloride-35Cl, 99 atom % 35Cl
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Sodium chloride, random crystals, 99.9% trace metals basis
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Acetone, natural, ≥97%
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Methanol, suitable for NMR (reference standard)
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Formaldehyde-12C solution, 20% in H2O, 99.9 atom % 12C
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Piperidine, ≥99.5%, purified by redistillation
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2-Mercaptoethanol, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
Supelco
Formaldehyde solution, stabilized with methanol, ~37 wt. % in H2O, certified reference material
Sigma-Aldrich
2-Mercaptoethanol, ≥99.0%
Sigma-Aldrich
Piperidine, ReagentPlus®, 99%
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Diethylene glycol, BioUltra, ≥99.0% (GC)
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2-Mercaptoethanol, BioUltra, Molecular Biology, ≥99.0% (GC)
SAFC
Sodium chloride solution, 5 M
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Tetrahydrofuran, inhibitor-free, purification grade
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Methanol, purification grade, 99.8%
Supelco
1-Octanol, analytical standard