Skip to Content

CN

Polymer enzyme conjugates as chiral ligands for sharpless dihydroxylation of alkenes in organic solvents.

Polymer enzyme conjugates as chiral ligands for sharpless dihydroxylation of alkenes in organic solvents.

Chembiochem : a European journal of chemical biology (2014-11-26)

Stefan Konieczny, Melanie Leurs, Joerg C Tiller

ABSTRACT

Conjugates of enzymes and poly(2-methyloxazoline) were used as organosoluble amphiphilic polymer nanocontainers for dissolving osmate, thereby converting the enzymes into organosoluble artificial metalloenzymes. These were shown to catalyze the dihydroxylation of different alkenes with high enantioselectivity. The highest selectivities, found for osmate complexed with laccase polymer-enzyme conjugates (PECs), even exceed those of classical Sharpless catalysts.

MATERIALS

Product Number

Brand

Product Description

Supelco

Ethanol solution, certified reference material, 2000 μg/mL in methanol

Supelco

Pyrogallol, analytical standard

Sigma-Aldrich

Pyrocatechol, ≥99%

Sigma-Aldrich

Chloroform, ≥99%, PCR Reagent, contains amylenes as stabilizer

Supelco

Ethanol standards 10% (v/v), 10 % (v/v) in H₂O, analytical standard

Supelco

Methanol, analytical standard

Supelco

Chloroform, analytical standard

Sigma-Aldrich

Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)

Sigma-Aldrich

Pyrogallol, ≥98% (HPLC)

Sigma-Aldrich

Methanol, anhydrous, 99.8%

Sigma-Aldrich

Pyrocatechol, purified by sublimation, ≥99.5%

Sigma-Aldrich

Pyrogallol, ACS reagent

Sigma-Aldrich

Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer

Sigma-Aldrich

Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%

Sigma-Aldrich

Pyrogallol, ACS reagent, ≥99%

Sigma-Aldrich

Methanol, purification grade, 99.8%

Supelco

Dehydrated Alcohol, Pharmaceutical Secondary Standard; Certified Reference Material

Sigma-Aldrich

Methanol, suitable for NMR (reference standard)

Sigma-Aldrich

Methanol, suitable for HPLC, gradient grade, ≥99.9%

Sigma-Aldrich

Chloroform, contains ethanol as stabilizer, ACS reagent, ≥99.8%

Sigma-Aldrich

Methanol, BioReagent, ≥99.93%

Sigma-Aldrich

Ethyl alcohol, Pure 190 proof, for molecular biology

Sigma-Aldrich

Chloroform, contains 100-200 ppm amylenes as stabilizer, ≥99.5%

Sigma-Aldrich

Methanol, suitable for HPLC, gradient grade, suitable as ACS-grade LC reagent, ≥99.9%

Supelco

Methanol, Pharmaceutical Secondary Standard; Certified Reference Material

Sigma-Aldrich

Pyrogallol, Vetec^™, reagent grade

Sigma-Aldrich

Methanol, ACS spectrophotometric grade, ≥99.9%

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

Sigma-Aldrich

Methanol, Laboratory Reagent, ≥99.6%

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%