New monoterpene glycosides from sunflower seeds and their protective effects against H2O2-induced myocardial cell injury.

New monoterpene glycosides from sunflower seeds and their protective effects against H2O2-induced myocardial cell injury.

Food chemistry (2015-05-16)

Yonghe Fei, Jianping Zhao, Yanli Liu, Xiaoran Li, Qiongming Xu, Taoyun Wang, Ikhlas A Khan, Shilin Yang

ABSTRACT

Three new monoterpene glycosides (1-3) and eleven known compounds (4-14) were isolated from seeds of Helianthus annuus L. (sunflower). Their structures were determined by spectroscopic and chemical methods. All the compounds were isolated from sunflower seeds for the first time. Protective effects of compounds 1-14 against H2O2-induced H9c2 cardiomyocyte injury were evaluated, and compounds 1 and 2 showed some cell-protective effects. No significant DPPH radical scavenging activity was observed for compounds 1-14.

MATERIALS

Product Number

Brand

Product Description

644374

Sigma-Aldrich

Methanol, purification grade, 99.8%

322415

Sigma-Aldrich

Methanol, anhydrous, 99.8%

523771

Sigma-Aldrich

Tetramethylsilane, electronic grade, ≥99.99% trace metals basis

87921

Sigma-Aldrich

Tetramethylsilane, ≥99.0% (GC)

T3251

Sigma-Aldrich

(±)-α-Tocopherol, synthetic, ≥96% (HPLC)

717975

Sigma-Aldrich

Methanol, suitable for NMR (reference standard)

258024

Sigma-Aldrich

α-Tocopherol, ≥95.5%

T1539

Sigma-Aldrich

(+)-α-Tocopherol, Type VI, from vegetable oil, liquid (≥0.88M based on potency, density and molecular wt.), BioReagent, suitable for insect cell culture, ≥1000 IU/g

T3634

Sigma-Aldrich

(+)-α-Tocopherol, from vegetable oil, Type V, ~1000 IU/g

281689

Sigma-Aldrich

1,1-Diphenyl-2-picrylhydrazine, 97%

633240

Sigma-Aldrich

Methanol solution, contains 0.50 % (v/v) triethylamine