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Analysis of Major Withanolides in Physalis longifolia Nutt. by HPLC-PDA.

Analysis of Major Withanolides in Physalis longifolia Nutt. by HPLC-PDA.

Journal of chromatographic science (2014-12-04)

Cong-Mei Cao, Kelly Kindscher, Robert J Gallagher, Huaping Zhang, Barbara N Timmermann

ABSTRACT

An analytical method based on high-performance liquid chromatography-photodiode array detection was developed for the simultaneous determination of three anti-proliferative withanolides [withalongolide A ( 1: ), withaferin A ( 2: ) and withalongolide B ( 3: )] present in the aboveground biomass of the long-leaf groundcherry, Physalis longifolia. This method was achieved by biomass extraction followed by chromatographic separation on C18 column eluted with a gradient acetonitrile-water mobile phase. Calibration curves produced satisfactory linear regression (r(2) > 0.9995) for each examined sample. The method was also validated for accuracy, precision and limits of detection and quantification. Such an approach is applicable for the rapid detection and quantitative assessment of withanolides in various P. longifolia accessions.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

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Methanol, ACS reagent, ≥99.8%

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Methanol, ACS spectrophotometric grade, ≥99.9%

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Methanol, BioReagent, ≥99.93%

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Methanol, puriss., meets analytical specification of Ph Eur, ≥99.7% (GC)

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Methanol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)

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Methanol, Laboratory Reagent, ≥99.6%

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Methanol, anhydrous, 99.8%

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Methanol, purification grade, 99.8%

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Methanol, suitable for NMR (reference standard)

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Methanol, ACS reagent, ≥99.8%

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Methanol solution, contains 0.50 % (v/v) triethylamine

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Dichloromethane, puriss., meets analytical specification of Ph. Eur., NF, ≥99% (GC)

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Hexane, Laboratory Reagent, ≥95%

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Hexane, ReagentPlus^®, ≥99%

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Acetonitrile, ReagentPlus^®, 99%

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Dichloromethane, ACS reagent, ≥99.5%, contains 40-150 ppm amylene as stabilizer

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Dichloromethane, ACS reagent, ≥99.5%, contains 40-150 ppm amylene as stabilizer

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Acetonitrile, ACS reagent, ≥99.5%

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Dichloromethane, contains 40-150 ppm amylene as stabilizer, ACS reagent, ≥99.5%

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Dichloromethane, suitable for HPLC, ≥99.9%, contains 40-150 ppm amylene as stabilizer

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Acetonitrile, anhydrous, 99.8%

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Dichloromethane, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer

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Ethyl acetate, ReagentPlus^®, ≥99.8%

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Ethyl acetate, anhydrous, 99.8%

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Ethyl acetate, puriss., meets analytical specification of Ph. Eur., BP, NF, ≥99.5% (GC)

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Ethyl acetate, ACS reagent, ≥99.5%

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Ethyl acetate, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)

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Ethyl acetate, biotech. grade, ≥99.8%

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