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  • N-linked glycosylation of a subunit isoforms is critical for vertebrate vacuolar H

N-linked glycosylation of a subunit isoforms is critical for vertebrate vacuolar H

Journal of cellular biochemistry (2017-07-01)
Sally Esmail, Norbert Kartner, Yeqi Yao, Joo Wan Kim, Reinhart A F Reithmeier, Morris F Manolson
ABSTRACT

The a subunit of the V

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
DL-Glyceraldehyde 3-phosphate solution, 45-55 mg/mL in H2O
Sigma-Aldrich
Phenylmethanesulfonyl fluoride, ≥98.5% (GC)
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Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
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Anti-VMA21 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution