A validated LC-MS/MS method for the determination of tolterodine and its metabolite in rat plasma and application to pharmacokinetic study.

A validated LC-MS/MS method for the determination of tolterodine and its metabolite in rat plasma and application to pharmacokinetic study.

Journal of pharmaceutical analysis (2013-12-01)

Rihana Parveen Shaik, Srinivasa Babu Puttagunta, Chandrasekhar Bannoth Kothapalli, Bahlul Zayed Sh Awen, B R Challa

ABSTRACT

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used for simultaneous quantification of tolterodine and its metabolite 5-hydroxy methyl tolterodine in rat plasma. Tolterodine-d6 and 5-hydroxy methyl tolterodine-d14 were used as internal standards (IS). Chromatographic separation was performed on Ascentis Express RP amide (50 mm×4.6 mm, 2.7 μm) column with an isocratic mobile phase composed of 10 mM ammonium acetate and acetonitrile in the ratio of 20:80 (v/v), at a flow-rate of 0.5 mL/min. Tolterodine, tolterodine-d6, 5-hydroxy methyl tolterodine and 5-hydroxy methyl tolterodine-d14 were detected with proton adducts at

MATERIALS

Product Number

Brand

Product Description

53514-U

Supelco

Ascentis^® Express RP-Amide, 2.7 μm Guard Cartridge, 2.7 μm particle size, L × I.D. 5 mm × 2.1 mm, pkg of 3 ea

53519-U

Supelco

Ascentis^® Express RP-Amide, 2.7 μm Guard Cartridge, 2.7 μm particle size, L × I.D. 5 mm × 4.6 mm, pkg of 3 ea

53516-U

Supelco

Ascentis^® Express RP-Amide, 2.7 μm Guard Cartridge, 2.7 μm particle size, L × I.D. 5 mm × 3 mm, pkg of 3 ea

565303-U

Supelco

Ascentis^® RP-Amide HPLC Column, 5 μm particle size, L × I.D. 5 cm × 2.1 mm

565348-U

Supelco

Ascentis^® RP-Amide HPLC Column, 5 μm particle size, L × I.D. 25 cm × 21.2 mm

565324-U

Supelco

Ascentis^® RP-Amide HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

565322-U

Supelco

Ascentis^® RP-Amide HPLC Column, 3 μm particle size, L × I.D. 15 cm × 4.6 mm

565320-U

Supelco

Ascentis^® RP-Amide HPLC Column, 3 μm particle size, L × I.D. 5 cm × 4.6 mm

565347-U

Supelco

Ascentis^® RP-Amide HPLC Column, 5 μm particle size, L × I.D. 15 cm × 21.2 mm

50767-U

Supelco

Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 3 cm × 4.6 mm

51577-U

Supelco

Ascentis^® Express RP-Amide, 2 μm UHPLC Column, 2 μm particle size, L × I.D. 15 cm × 2.1 mm

50732-U

Supelco

Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 2 cm × 2.1 mm

50733-U

Supelco

Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 3 cm × 2.1 mm

51583-U

Supelco

Ascentis^® Express RP-Amide, 2 μm UHPLC Column, 2 μm particle size, L × I.D. 5 cm × 3 mm

51582-U

Supelco

Ascentis^® Express RP-Amide, 2 μm UHPLC Column, 2 μm particle size, L × I.D. 3 cm × 3 mm

51589-U

Supelco

Ascentis^® Express RP-Amide, 2 μm UHPLC Column, 2 μm particle size, L × I.D. 15 cm × 3 mm

51588-U

Supelco

Ascentis^® Express RP-Amide, 2 μm UHPLC Column, 2 μm particle size, L × I.D. 10 cm × 3 mm

51567-U

Supelco

Ascentis^® Express RP-Amide, 2 μm UHPLC Column, 2 μm particle size, L × I.D. 2 cm × 2.1 mm

51568-U

Supelco

Ascentis^® Express RP-Amide, 2 μm UHPLC Column, 2 μm particle size, L × I.D. 3 cm × 2.1 mm

51569-U

Supelco

Ascentis^® Express RP-Amide, 2 μm UHPLC Column, 2 μm particle size, L × I.D. 5 cm × 2.1 mm

51571-U

Supelco

Ascentis^® Express RP-Amide, 2 μm UHPLC Column, 2 μm particle size, L × I.D. 7.5 cm × 2.1 mm

50739-U

Supelco

Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm

50747-U

Supelco

Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 25 cm × 2.1 mm

50773-U

Supelco

Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 10 cm × 4.6 mm

50749-U

Supelco

Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 3 cm × 3 mm

50737-U

Supelco

Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 10 cm × 2.1 mm

50774-U

Supelco

Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

50777-U

Supelco

Ascentis^® Express RP-Amide, 5 μm Guard Cartridge, 5 μm particle size, L × I.D. 5 mm × 3 mm, pkg of 3 ea

50751-U

Supelco

Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 5 cm × 3 mm

50775-U

Supelco

Ascentis^® Express RP-Amide, 5 μm HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm