565325-U
Ascentis® RP-Amide HPLC Column
5 μm particle size, L × I.D. 25 cm × 4.6 mm
material
stainless steel column
Quality Level
Agency
suitable for USP L60
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
extent of labeling
19.5% Carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
25 cm × 4.6 mm
surface area
450 m2/g
surface coverage
2.7 μmol/m2
impurities
<5 ppm metals
matrix
fully porous particle
silica gel high purity, spherical
matrix active group
amide, alkyl phase
particle size
5 μm
pore size
100 Å
operating pH range
2-8
application(s)
environmental
food and beverages
separation technique
reversed phase
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General description
Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.
Features and Benefits
- Excellent retention and peak shape for polar compounds
- 100% aqueous compatibility
- Ultra low bleed, LC-MS compatible
- Unique selectivity
Legal Information
guard cartridge
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.
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Example:
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705578-5MG-PW
PL860-CGA/SHF-1EA
MMYOMAG-74K-13
1000309185
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How to Find a Lot/Batch Number for COA
Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.
Aldrich Products
For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').
For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').
For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.
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In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Can I use Ascentis Express on a UHPLC system?
Yes. Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
What solvent should Product No. 565325-U Ascentis RP-Amide HPLC Column be stored in?
Product No. 565325-U should be stored in 100% acetonitrile after any buffers have been flushed out.
What is the Product No of the guard column for Product No. 565325-U Ascentis RP-Amide HPLC column?
The guard column kit for Product No. 565325-U is 565371-U. This kit includes one cartridge, stand-alone holder, a piece of tubing, 2 nuts and 2 ferrules. A set of two replacement cartridges is Product No. 565370-U.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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