2-D Quant Kit is designed to accurately determine protein concentrations in samples for electrophoresis. Proteins are quantitatively precipitated while interfering substances are left in solution. The color density that develops in the 2-D Quant Kit procedure is inversely related to the protein concentration, with a linear response to protein in the range of 0–50 µg and a volume range of 1–50 µL. The procedure is compatible with common sample preparation reagents listed in Table 13.
Figure 15.The 2-D Quant Kit protein assay eliminates interference from sample solution components
Components supplied
Precipitant, co-precipitant, copper solution, color reagent A, color reagent B, bovine serum albumin (BSA) standard solution.
Required but not provided
Microcentrifuge, microcentrifuge tubes (2 mL), vortex mixer, visible-light spectrophotometer, spectrophotometer cells. Preliminary notes Prior to starting procedure, prepare working color reagent by mixing 100 parts of color reagent A with one part of color reagent B. Each individual assay requires 1 mL working color reagent.
Proceed rapidly to avoid resuspension or dispersion of pellet. There should be no visible liquid remaining.
Ensure that the pellet is completely resuspended by vortexing thoroughly.
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