CN
Dot/Slot (Filtration) Blotting
Dot blotting is an ideal technique for quickly assessing the levels of a target antigen across many samples at once. Also, it is a popular method for epitope mapping and screening antibodies for target specificity.
Dot/Slot (Filtration) blotting topics: possible causes and remedies:
Figure 1.Detection of transferrin on a dot blot of human serum using Immobilon® Western Chemiluminescent HRP Substrate.
Slow or No Filtration of the Sample Through the Membrane |
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Little or No Protein Observed on the Blot |
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Stained Blot Is Not Uniform |
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Protein Smeared Across the Top of the Membrane |
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Protein Smeared Across the Back of the Membrane |
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Materials
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