Dot/Slot (Filtration) Blotting

Dot blotting is an ideal technique for quickly assessing the levels of a target antigen across many samples at once. Also, it is a popular method for epitope mapping and screening antibodies for target specificity.

Dot/Slot (Filtration) blotting topics: possible causes and remedies:

• Slow or No Filtration of the Sample Through the Membrane
• Little or No Protein Observed on the Blot
• Stained Blot Is Not Uniform
• Protein Smeared Across the Top of the Membrane
• Protein Smeared Across the Back of the Membrane