biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CENPA(1058)
General description
17kDa
CENP-A是仅在着丝粒处发现的组蛋白H3的变体形式。
着丝粒蛋白A（CENP-A）是一种17 kDa着丝粒特异性组蛋白变体，与组蛋白H3的C端具有62％的氨基酸同源性。它位于着丝粒中，在着丝粒特异性染色质的形成中起着关键作用。敲除组蛋白H3的CENP-A结合域的研究表明，CENP-A可能是将α-卫星DNA包装到初级染色结构中的组蛋白H3的替代品。CENP-A是着丝粒的特异性核小体的形成所必需的，这暗示着CENP-A是着丝粒特异性的表观遗传标记。
Immunogen
对应于人CENP-A氨基酸4-17的肽（RRRSRKPEAPRRRS）。
Application
抗CENP-A抗体是用于检测CENP-A（也称为着丝粒自身抗原A）的兔多克隆抗体 & 在WB中进行了测试。
研究子类别
细胞周期，DNA 复制&修复
细胞周期，DNA 复制&修复
研究类别
表观遗传学&核功能
表观遗传学&核功能
Biochem/physiol Actions
CENP-A
预计具有广泛的物种交叉反应性
Physical form
亲和纯化的免疫球蛋白，溶于0.1 Tris-甘氨酸（pH 7.4）、0.15 NaCl和0.05％叠氮化钠中。
免疫亲和纯化
Preparation Note
自收到之日起，在2至8ºC条件下可稳定保存1年。
Analysis Note
对照
秋水仙酰胺处理或未处理的对照组蛋白（来自HeLa细胞）
秋水仙酰胺处理或未处理的对照组蛋白（来自HeLa细胞）
通过免疫印迹对HeLa细胞的酸提取组蛋白进行了常规评估
Other Notes
替代：04-205
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 1
Qubo Zhu et al.
The Journal of cell biology, 185(5), 827-839 (2009-06-03)
Telomeric repeat binding factor 1 (TRF1) is a component of the multiprotein complex "shelterin," which organizes the telomere into a high-order structure. TRF1 knockout embryos suffer from severe growth defects without apparent telomere dysfunction, suggesting an obligatory role for TRF1
CENP-A nucleosomes localize to transcription factor hotspots and subtelomeric sites in human cancer cells.
Athwal, RK; Walkiewicz, MP; Baek, S; Fu, S; Bui, M; Camps, J; Ried, T; Sung, MH; Dalal, Y
Epigenetics & Chromatin null
The histone H3/H4 chaperone CHAF1B prevents the mislocalization of CENP-A for chromosomal stability.
Roshan L Shrestha et al.
Journal of cell science, 136(10) (2023-05-02)
Restricting the localization of the evolutionarily conserved centromeric histone H3 variant CENP-A to centromeres prevents chromosomal instability (CIN). The mislocalization of CENP-A to non-centromeric regions contributes to CIN in yeasts, flies and human cells. Even though overexpression and mislocalization of
Yujue Chen et al.
The Journal of cell biology, 220(7) (2021-04-22)
Centromeric transcription has been shown to play an important role in centromere functions. However, lack of approaches to specifically manipulate centromeric transcription calls into question that the proposed functions are a direct consequence of centromeric transcription. By monitoring nascent RNAs
Qinfu Chen et al.
Cell reports, 36(12), 109740-109740 (2021-09-23)
Bub1 is required for the kinetochore/centromere localization of two essential mitotic kinases Plk1 and Aurora B. Surprisingly, stable depletion of Bub1 by ∼95% in human cells marginally affects whole chromosome segregation fidelity. We show that CENP-U, which is recruited to
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