Merck
CN

1.10338

Millipore

Fractogel EMD螯合物(M)

purified by immobilized metal affinity chromatography (IMAC)

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别名:
Fractogel® EMD Chelate (M)

ligand

iminodiacetic acid

质量水平

描述

for metal affinity chromatography (IMAC), suspension in 20% ethanol and 150 mM NaCl (40-90 µm)

无菌性

sterile (Caustic Stable)

形式

resin

纯化方式

immobilized metal affinity chromatography (IMAC)

manufacturer/tradename

Fractogel®

参数

8 bar max. pressure
~240 cm/hr flow rate

基质活性基团

methacrylate

wide

40-90 μm

容量

~60 mg binding capacity( lysozyme/ml of resin)

转变温度

flash point 35 °C (calculated)

application(s)

recombinant protein

分离技术

affinity

储存温度

2-30°C

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此商品
1.168841.168821.16886
Fractogel® EMD Chelate (M) purified by immobilized metal affinity chromatography (IMAC)

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Fractogel® EMD SO3 ⁻ (M) Resin

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Fractogel EMD SO₃⁻ (M)

Fractogel® EMD COO- (M)

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Fractogel EMD COO⁻ (M)

manufacturer/tradename

Fractogel®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

parameter

8 bar max. pressure, ~240 cm/hr flow rate

parameter

170 cm/hr flow rate, 8 bar max. pressure

parameter

8 bar max. pressure

parameter

300 cm/hr flow rate, 8 bar max. pressure

matrix active group

methacrylate

matrix active group

methacrylate

matrix active group

methacrylate

matrix active group

methacrylate

capacity

~60 mg binding capacity( lysozyme/ml of resin)

capacity

100 mg binding capacity (BSA/mg of resin)

capacity

130 mg binding capacity (lysozyme/ml of resin)

capacity

100 mg binding capacity (lysozyme/ml of resin)

separation technique

affinity

separation technique

weak anion exchange

separation technique

cation exchange, strong cation exchange

separation technique

cation exchange, weak cation exchange

一般描述

Fractogel® EMD Chelate combines our tentacle structure with iminodiacetic acid as the metal chelating ligand

特点和优势

Fractogel® EMD Chelate (M) enables users to:

  • Efficiently purify histidine-tagged recombinant proteins
  • Coordinate metal ions to non-bonding electron pairs of the amino acid side chains and separate peptides
  • Ensure timely and successful startup and operation
  • Minimize hydrophobic interactions due to the tentacles technology, resulting in superior selectivity and recovery
  • Handle multiple CIP and packing cycles with the chemically and physically robust bead, leading to a longer resin lifetime

包装

  • 1.10338.0100: Fractogel® EMD Chelate (M) Resin 100ml
  • 1.10338.0010: Fractogel® EMD Chelate (M) Resin 10 ml
  • 1.10338.0500: Fractogel® EMD Chelate (M) Resin 500 ml
  • 1.10338.5000: Fractogel® EMD Chelate (M) Resin 5 L

Plastic bottle

分析说明

Appearance: Milky tubid suspension, free from impurities (foreigen particles)
Microscopic evaluation: Uniform spherical particles, no agglomerates, no fines
Cerium: ≤ 10 µg/g
Copper-(II)-binding capacity: 60 - 100 µmol/ml
Extractable matter (water): ≤ 0.05 %
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Functional test: passes test
Functional test: Separation of chymotrypsinogen A and myoglobin

法律信息

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Flame

警示用语:

Warning

危险声明

危险分类

Flam. Liq. 3

储存分类代码

3 - Flammable liquids

WGK

WGK 1

闪点(°F)

95.0 °F

闪点(°C)

35 °C


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