ligand
iminodiacetic acid
Quality Segment
form
resin
purified by
immobilized metal affinity chromatography (IMAC)
manufacturer/tradename
Fractogel®
parameter
8 bar max. pressure, ~240 cm/hr flow rate
matrix active group
methacrylate
wide
40-90 μm
capacity
~60 mg binding capacity( lysozyme/ml of resin)
transition temp
flash point 35 °C (calculated)
application(s)
recombinant protein
separation technique
affinity
storage temp.
2-30°C
General description
Fractogel® EMD Chelate combines our tentacle structure with iminodiacetic acid as the metal chelating ligand
Features and Benefits
Fractogel® EMD Chelate (M) enables users to:
- Efficiently purify histidine-tagged recombinant proteins
- Coordinate metal ions to non-bonding electron pairs of the amino acid side chains and separate peptides
- Ensure timely and successful startup and operation
- Minimize hydrophobic interactions due to the tentacles technology, resulting in superior selectivity and recovery
- Handle multiple CIP and packing cycles with the chemically and physically robust bead, leading to a longer resin lifetime
Packaging
- 1.10338.0100: Fractogel® EMD Chelate (M) Resin 100ml
- 1.10338.0010: Fractogel® EMD Chelate (M) Resin 10 ml
- 1.10338.0500: Fractogel® EMD Chelate (M) Resin 500 ml
- 1.10338.5000: Fractogel® EMD Chelate (M) Resin 5 L
Plastic bottle
Analysis Note
Appearance: Milky tubid suspension, free from impurities (foreigen particles)
Microscopic evaluation: Uniform spherical particles, no agglomerates, no fines
Cerium: ≤ 10 µg/g
Copper-(II)-binding capacity: 60 - 100 µmol/ml
Extractable matter (water): ≤ 0.05 %
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Functional test: passes test
Functional test: Separation of chymotrypsinogen A and myoglobin
Microscopic evaluation: Uniform spherical particles, no agglomerates, no fines
Cerium: ≤ 10 µg/g
Copper-(II)-binding capacity: 60 - 100 µmol/ml
Extractable matter (water): ≤ 0.05 %
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Functional test: passes test
Functional test: Separation of chymotrypsinogen A and myoglobin
Legal Information
FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany
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signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
存储类别
3 - Flammable liquids
wgk
WGK 1
flash_point_f
95.0 °F
flash_point_c
35 °C
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